1887

Abstract

A Gram-staining-positive, non-motile, strictly aerobic, non-spore-forming, vibrio-shaped bacterial strain, CL-TW6, was isolated from a reservoir seawater sample from a solar saltern in Korea. Analysis of the 16S rRNA gene sequence of strain CL-TW6 revealed a clear affiliation with the family . Strain CL-TW6 showed the closest phylogenetic relationships with the genera and , with 16S rRNA gene sequence similarity of 94.8–95.3 %. The strain grew in the presence of 1–9 % sea salts, at 15–35 °C and at pH 7.0–9.0. The major cellular fatty acids of strain CL-TW6 were anteiso-C (32.6 %), iso-C (20.4 %), iso-C (13.2 %) and iso-C (11.8 %) and the major menaquinones were MK-9 and MK-10. Cell-wall analysis showed that the peptidoglycan of strain CL-TW6 contained 2,4-diaminobutyric acid, alanine, glycine and glutamic acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content of strain CL-TW6 was 60.0 mol%. The combined phenotypic, chemotaxonomic and phylogenetic data showed clearly that strain CL-TW6 could be distinguished from members of the family with validly published names. Thus, strain CL-TW6 should be classified as representing a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is CL-TW6 ( = KCCM 90105  = JCM 18206).

Funding
This study was supported by the:
  • National Research Foundation of Korea (NRF)
  • Korean Government (MEST) (Award 2011-0012369)
  • Ministry of Land, Transport and Maritime Affairs, Korea
  • BK21 project of the Korean Government
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2013-06-01
2021-04-18
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