1887

Abstract

A novel filamentous bacterium, designated strain JIR-001, was isolated from hemipelagic sediment in deep seawater. This strain was non-motile, Gram-positive, aerobic, heterotrophic and thermophilic; colonies were of infinite form and ivory coloured with wrinkles between the centre and the edge of the colony on ISP2 medium. The isolate grew aerobically at 55–73 °C with the formation of aerial mycelia; spores were produced singly along the aerial mycelium. These morphological features show some similarities to those of the type strains of some species belonging to the family . Phylogenetic analysis based on 16S rRNA gene sequences confirmed that strain JIR-001 belongs to the family within the class . Similarity levels between the 16S rRNA gene sequence of strain JIR-001 and those of the type strains of species were 85.5–93.5 %; highest sequence similarity was with NariEX. In the DNA–DNA hybridization assays between strain JIR-001 and its phylogenetic neighbours the mean hybridization levels with NariEX, H0165, 500275 and LA5 were 5.3–7.5, 2.3–4.7, 2.1–4.8 and 2.5–4.9 %, respectively. The DNA G+C content of strain JIR-001 was 55.1 mol%. The major fatty acids were iso-C, iso-C, iso-C and C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, glucolipid, phosphatidylserine, an amino-group containing phospholipid, an unknown phospholipid and two unknown lipids. The predominant menaquinone was MK-7 and the cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid and alanine. On the basis of phenotypic characteristics and 16S rRNA gene sequence comparisons, strain JIR-001 is considered to represent a novel species in a new genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is JIR-001 ( = JCM 18147 = CECT 8074).

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2013-06-01
2019-12-07
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