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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_7

sp. nov., isolated from a root of Free

Abstract

A novel endophytic actinomycete, strain 274745, was isolated from a root of collected in a mangrove forest in Sanya, Hainan province, China. The 16S rRNA gene sequence of strain 274745 showed the greatest similarity to TJ2-2 (98.3 %). Phylogenetic analysis based on the gene also supported the close relationship of these two strains. The predominant menaquinone was MK-10(H) and the major fatty acids were iso-C, C and anteiso-C. The characteristic whole-cell sugars were xylose and mannose. The cell wall contained -diaminopimelic acid and glycine. The polar lipid profile mainly comprised phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The DNA G+C content was 71.6 mol%. Furthermore, a combination of DNA–DNA relatedness and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 274745 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 274745 ( = CCTCC AA 2012003  = DSM 45704).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31170467)
© 2013 IUMS
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2013-07-01
2025-04-22
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References

  1. Arai T. ( 1975 ). Culture Media for Actinomycetes. Tokyo:: The Society for Actinomycetes Japan;.
     [Google Scholar]
  2. Carro L., Pukall R., Spröer C., Kroppenstedt R. M., Trujillo M. E. ( 2012 ). Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum . . Int J Syst Evol Microbiol 62, 2971–2977. [View Article] [PubMed]
    [Google Scholar]
  3. Castiglione F., Lazzarini A., Carrano L., Corti E., Ciciliato I., Gastaldo L., Candiani P., Losi D., Marinelli F. & other authors ( 2008 ). Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens. . Chem Biol 15, 22–31. [View Article] [PubMed]
    [Google Scholar]
  4. Everest G. J., Meyers P. R. ( 2012 ). Micromonospora equina sp. nov., isolated from soil from a racecourse in South Africa. . Int J Syst Evol Microbiol 63, 879–886. [View Article] [PubMed]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  7. Garcia L. C., Martínez-Molina E., Trujillo M. E. ( 2010 ). Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum . . Int J Syst Evol Microbiol 60, 331–337. [View Article] [PubMed]
    [Google Scholar]
  8. Hong K., Gao A. H., Xie Q. Y., Gao H., Zhuang L., Lin H. P., Yu H. P., Li J., Yao X. S. & other authors ( 2009 ). Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. . Mar Drugs 7, 24–44. [View Article] [PubMed]
    [Google Scholar]
  9. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogeneous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141–145. [PubMed]
    [Google Scholar]
  10. Kämpfer P., Steiof M., Dott W. ( 1991 ). Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. . Microb Ecol 21, 227–251. [View Article]
    [Google Scholar]
  11. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  12. Kim B. Y., Stach J. E. M., Weon H. Y., Kwon S. W., Goodfellow M. ( 2010 ). Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil. . Int J Syst Evol Microbiol 60, 1813–1823. [View Article] [PubMed]
    [Google Scholar]
  13. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 1–32. [View Article]
    [Google Scholar]
  14. Kroppenstedt R. M. ( 1985 ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by Goodfellow M., Minnikin D. E. . New York:: Academic Press;.
     [Google Scholar]
  15. Lechevalier M. P., Lechevalier H. A. ( 1980 ). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 227–291. Edited by Dietz A., Thayer J. . Arlington, VA:: Society for Industrial Microbiology;.
     [Google Scholar]
  16. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977 ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5, 249–260. [View Article]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. K. ( 1984 ). An integrated procedure for the extraction of isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  19. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. ( 1999 ). Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. . Int J Syst Bacteriol 49, 1761–1767. [View Article] [PubMed]
    [Google Scholar]
  20. Ørskov J. ( 1923 ). Investigations into the Morphology of the Ray Fungi. Copenhagen:: Levin and Munksgaard;.
     [Google Scholar]
  21. Pospiech A., Neumann B. ( 1995 ). A versatile quick-prep of genomic DNA from gram-positive bacteria. . Trends Genet 11, 217–218. [View Article] [PubMed]
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425. [PubMed]
    [Google Scholar]
  23. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids . , MIDI Technical Note 101. Newark, DE:: MIDI Inc.;
    [Google Scholar]
  24. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  25. Songsumanus A., Tanasupawat S., Thawai C., Suwanborirux K., Kudo T. ( 2011 ). Micromonospora humi sp. nov., isolated from peat swamp forest soil. . Int J Syst Evol Microbiol 61, 1176–1181. [View Article] [PubMed]
    [Google Scholar]
  26. Songsumanus A., Tanasupawat S., Igarashi Y., Kudo T. ( 2013 ). Micromonospora maritima sp. nov., isolated from mangrove soil. . Int J Syst Evol Microbiol 63, 554–559. [View Article] [PubMed]
    [Google Scholar]
  27. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33, 152–155.
     [Google Scholar]
  28. Supong K., Suriyachadkun C., Tanasupawat S., Suwanborirux K., Pittayakhajonwut P., Kudo T., Thawai C. ( 2013 ). Micromonospora sediminicola sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 63, 570–575. [View Article] [PubMed]
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  30. Thawai C., Tanasupawat S., Kudo T. ( 2008 ). Micromonospora pattaloongensis sp. nov., isolated from a Thai mangrove forest. . Int J Syst Evol Microbiol 58, 1516–1521. [View Article] [PubMed]
    [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  32. Waksman S. A. ( 1967 ). The Actinomycetes. A Summary of Current Knowledge. New York:: Ronald Press;.
     [Google Scholar]
  33. Wang C., Xu X. X., Qu Z., Wang H. L., Lin H. P., Xie Q. Y., Ruan J. S., Hong K. ( 2011 ). Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. . Int J Syst Evol Microbiol 61, 320–324. [View Article] [PubMed]
    [Google Scholar]
  34. Williams S. T., Cross T. ( 1971 ). Actinomycetes. . In Methods in Microbiology, vol. 4, pp. 295–334. Edited by Booth C. . London:: Academic Press;.
     [Google Scholar]
  35. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 1743–1813. [PubMed]
    [Google Scholar]
  36. Xi L. J., Zhang L. M., Ruan J. S., Huang Y. ( 2011 ). Nonomuraea maritima sp. nov., isolated from coastal sediment. . Int J Syst Evol Microbiol 61, 2740–2744. [View Article] [PubMed]
    [Google Scholar]
  37. Xie Q. Y., Wang C., Wang R., Qu Z., Lin H. P., Goodfellow M., Hong K. ( 2011 ). Jishengella endophytica gen. nov., sp. nov., a new member of the family Micromonosporaceae . . Int J Syst Evol Microbiol 61, 1153–1159. [View Article] [PubMed]
    [Google Scholar]
  38. Xie Q. Y., Qu Z., Lin H. P., Li L., Hong K. ( 2012a ). Micromonospora haikouensis sp. nov., isolated from mangrove soil. . Antonie van Leeuwenhoek 101, 649–655. [View Article] [PubMed]
    [Google Scholar]
  39. Xie Q. Y., Lin H. P., Li L., Brown R., Goodfellow M., Deng Z. X., Hong K. ( 2012b ). Verrucosispora wenchangensis sp. nov., isolated from mangrove soil. . Antonie van Leeuwenhoek 102, 1–7. [View Article] [PubMed]
    [Google Scholar]
  40. Zhang L. M., Xi L. J., Ruan J. S., Huang Y. ( 2012 ). Micromonospora yangpuensis sp. nov., isolated from a sponge. . Int J Syst Evol Microbiol 62, 272–278. [View Article] [PubMed]
    [Google Scholar]
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Micromonospora sonneratiae sp. nov., isolated from a root of Sonneratia apetala
Int J Syst Evol Microbiol 63, 2383 (2013); https://doi.org/10.1099/ijs.0.043570-0
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