1887

Abstract

A novel endophytic actinomycete, strain 274745, was isolated from a root of collected in a mangrove forest in Sanya, Hainan province, China. The 16S rRNA gene sequence of strain 274745 showed the greatest similarity to TJ2-2 (98.3 %). Phylogenetic analysis based on the gene also supported the close relationship of these two strains. The predominant menaquinone was MK-10(H) and the major fatty acids were iso-C, C and anteiso-C. The characteristic whole-cell sugars were xylose and mannose. The cell wall contained -diaminopimelic acid and glycine. The polar lipid profile mainly comprised phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The DNA G+C content was 71.6 mol%. Furthermore, a combination of DNA–DNA relatedness and some physiological and biochemical properties indicated that the novel strain could be readily distinguished from the closest phylogenetic relatives. On the basis of these phenotypic and genotypic data, strain 274745 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 274745 ( = CCTCC AA 2012003  = DSM 45704).

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2013-07-01
2019-10-14
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