1887

Abstract

A novel filamentous bacterial strain, HSS8-18, was isolated from soil from a hot-spring pond in Krabi province, Thailand. The isolate produced single globose bodies and short, finger-like sporangia directly from the substrate mycelium. The cell wall of the isolate contained -diaminopimelic acid, glycine, arabinose and xylose. The characteristic phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, phosphatidylethanolamine and hydroxyl phosphatidylethanolamine. The predominant menaquinones were MK-10(H), MK-9(H) and MK-10(H) and the predominant cellular fatty acids were anteiso-C, iso-C, iso-C, iso-C and anteiso-C. The G+C content of the genomic DNA was 72.0 mol%. 16S rRNA gene sequence analysis indicated that isolate HSS8-18 belonged to the genus and shared the highest 16S rRNA gene sequence similarity with YIM 46034 (98.3 %) and YIM 48875 (98.0 %). However, DNA–DNA hybridization results and some physiological and biochemical properties indicated that strain HSS8-18 could be readily distinguished from its closest phylogenetic relatives. Based on phenotypic and genotypic properties, isolate HSS8-18 is judged to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain HSS8-18 ( = BCC 41917  = JCM 17129).

Funding
This study was supported by the:
  • King Mongkut’s Institute of Technology Ladkrabang Research Fund
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2013-03-01
2024-04-24
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References

  1. Arai T. ( 1975 ). Culture Media for Actinomycetes. Tokyo: The Society for Actinomycetes (in Japanese)..
    [Google Scholar]
  2. Cao Y. R., Wang Q., Jin R. X., Jiang Y., Lai H. X., He W. X., Xu L. H., Jiang C. L. ( 2011 ). Planosporangium mesophilum sp. nov., isolated from rhizosphere soil of Bletilla striata . . Int J Syst Evol Microbiol 61, 13301333. [View Article] [PubMed]
    [Google Scholar]
  3. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [View Article] [PubMed]
    [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  5. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  7. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  8. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 5463. [View Article]
    [Google Scholar]
  9. Hayakawa M., Nonomura H. ( 1987 ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. . J Ferment Technol 65, 501509. [View Article]
    [Google Scholar]
  10. Itoh T., Kudo T., Parenti F., Seino A. ( 1989 ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. . Int J Syst Bacteriol 39, 168173. [View Article]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [View Article]
    [Google Scholar]
  12. Kawamoto I., Oka T., Nara T. ( 1981 ). Cell wall composition of Micromonospora olivasterospora, Micromonospora sagamiensis, and related organisms. . J Bacteriol 146, 527534.[PubMed]
    [Google Scholar]
  13. Kelly K. L. ( 1964 ). Inter-Society Color Council–National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  15. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  17. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115148. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  18. Leifson E. ( 1960 ). Atlas of Bacterial Flagellation. London:: Academic Press;.
    [Google Scholar]
  19. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  20. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. ( 1999 ). Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. . Int J Syst Bacteriol 49, 17611767. [View Article] [PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc.;
  23. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [View Article]
    [Google Scholar]
  24. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226231.[PubMed]
    [Google Scholar]
  25. Tamaoka J. ( 1994 ). Determination of DNA base composition. . In Chemical Methods in Prokaryotic Systematics, pp. 463470. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  26. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  28. Thawai C., Tanasupawat S., Itoh T., Suwanborirux K., Kudo T. ( 2005 ). Micromonospora siamensis sp. nov., isolated from Thai peat swamp forest. . J Gen Appl Microbiol 51, 229234. [View Article] [PubMed]
    [Google Scholar]
  29. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  30. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  31. Uchida K., Aida K. ( 1984 ). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. . J Gen Appl Microbiol 30, 131134. [View Article]
    [Google Scholar]
  32. Verlander C. P. ( 1992 ). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185201. Edited by Kricka L. J. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  33. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  34. Wiese J., Jiang Y., Tang S. K., Thiel V., Schmaljohann R., Xu L. H., Jiang C. L., Imhoff J. F. ( 2008 ). A new member of the family Micromonosporaceae, Planosporangium flavigriseum gen. nov., sp. nov.. Int J Syst Evol Microbiol 58, 13241331. [View Article] [PubMed]
    [Google Scholar]
  35. Williams S. T., Cross T. ( 1971 ). Actinomycetes. . Methods Microbiol 4, 295334. [View Article]
    [Google Scholar]
  36. Zhi X. Y., Li W. J., Stackebrandt E. ( 2009 ). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59, 589608. [View Article] [PubMed]
    [Google Scholar]
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