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Abstract

A hyperthermophilic anaerobic bacterium, designated D2C22, was isolated from the hydrothermal hot spring of Guelma in north-east Algeria. The isolate was a Gram-stain-positive, non-sporulating, non-motile rod, appearing singly or in pairs (0.3–0.4×8.0–9.0 µm). Strain D2C22 grew anaerobically at 45–85 °C (optimum 65 °C), at pH 5–9 (optimum pH 6.8) and with 0–20 g NaCl l. Strain D2C22 used glucose, galactose, lactose, fructose, ribose, xylose, arabinose, maltose, cellobiose, mannose, melibiose, sucrose, xylan and pyruvate (only in the presence of yeast extract or biotrypticase) as electron donors. The end products from glucose fermentation were acetate, lactate, CO and H. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate and sulfite were not used as electron acceptors. The predominant cellular fatty acids were iso-C and iso-C. The DNA G+C content was 41.6 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain D2C22 was most closely related to JW/HY-331, TH7C1 and DSM 20678 (95.5, 95.5 and 95.3 % 16S rRNA gene sequence similarity, respectively). Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain D2C22 is proposed to be a representative of a novel species of the genus within the order , for which the name sp. nov. is proposed. The type strain is D2C22 ( = DSM 24605 = JCM 17646).

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2013-06-01
2020-01-22
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Balch W. E. , Fox G. E. , Magrum L. J. , Woese C. R. , Wolfe R. S. . ( 1979; ). Methanogens: reevaluation of a unique biological group. . Microbiol Rev 43:, 260–296.[PubMed]
    [Google Scholar]
  3. Ben Dhia Thabet O. , Fardeau M.-L. , Joulian C. , Thomas P. , Hamdi M. , Garcia J.-L. , Ollivier B. . ( 2004; ). Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse. . Anaerobe 10:, 185–190. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bouanane-Darenfed A. , Fardeau M.-L. , Grégoire P. , Joseph M. , Kebbouche-Gana S. , Benayad T. , Hacene H. , Cayol J.-L. , Ollivier B. . ( 2011; ). Caldicoprobacter algeriensis sp. nov. a new thermophilic anaerobic, xylanolytic bacterium isolated from an Algerian hot spring. . Curr Microbiol 62:, 826–832. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–787. [CrossRef]
    [Google Scholar]
  7. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  8. Hungate R. E. . ( 1969; ). A roll -tube method for cultivation of strict anaerobes. . In Methods in Microbiology, vol. 3B, pp. 117–132. Edited by Norris J. R. , Ribbons D. W. . . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  9. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  10. Kuykendall L. D. , Roy M. A. , O’Neill J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and desoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  11. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C. content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  12. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  13. Rhuland L. E. , Work E. , Denman R. F. , Hoare D. S. . ( 1955; ). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77:, 4844–4846. [CrossRef]
    [Google Scholar]
  14. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  15. Schumann P. . ( 2011; ). Peptidoglycan structure. . In Methods in Microbiology, vol. 38, pp. 101–129. Edited by Rainey F. , Oren A. . . Chennay:: Academic Press;.
    [Google Scholar]
  16. Winker S. , Woese C. R. . ( 1991; ). A definition of the domains Archaea, Bacteria and Eucarya in terms of small subunit ribosomal RNA characteristics. . Syst Appl Microbiol 14:, 305–310. [CrossRef] [PubMed]
    [Google Scholar]
  17. Winter J. , Braun E. , Zabel H. P. . ( 1987; ). Acetomicrobium faecalis spec. nov., a strictly anaerobic bacterium from sewage sludge, producing ethanol from pentoses. . Syst Appl Microbiol 9:, 71–76. [CrossRef]
    [Google Scholar]
  18. Yokoyama H. , Wagner I. D. , Wiegel J. . ( 2010; ). Caldicoprobacter oshimai gen. nov., sp. nov., an anaerobic, xylanolytic, extremely thermophilic bacterium isolated from sheep faeces, and proposal of Caldicoprobacteraceae fam. nov.. Int J Syst Evol Microbiol 60:, 67–71. [CrossRef] [PubMed]
    [Google Scholar]
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