1887

Abstract

The taxonomic status of 14 strains of members of the genus isolated from floral nectar of wild Mediterranean insect-pollinated plants, which did not belong to any previously described species within this genus, was investigated following a polyphasic approach. Confirmation that these strains formed two separate lineages within the genus was obtained from comparative analysis of the partial sequences of the 16S rRNA gene and the gene encoding the β-subunit of RNA polymerase (), DNA–DNA reassociation data, determination of the DNA G+C content and physiological tests. The names sp. nov. and sp. nov. are proposed. The type strain of sp. nov. is SAP 763.2 ( = LMG 26958 = CECT 8127) and that of sp. nov. is SAP 284.1 ( = LMG 26959 = CECT 8128).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043489-0
2013-04-01
2019-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/4/1532.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043489-0&mimeType=html&fmt=ahah

References

  1. Álvarez-Pérez S. , Herrera C. M. . ( 2013; ). Composition, richness and nonrandom assembly of culturable bacterial–microfungal communities in floral nectar of Mediterranean plants. . FEMS Microbiol Ecol 83:, 685–699. [CrossRef] [PubMed]
    [Google Scholar]
  2. Álvarez-Pérez S. , Herrera C. M. , de Vega C. . ( 2012; ). Zooming-in on floral nectar: a first exploration of nectar-associated bacteria in wild plant communities. . FEMS Microbiol Ecol 80:, 591–602. [CrossRef] [PubMed]
    [Google Scholar]
  3. Anandham R. , Weon H. Y. , Kim S. J. , Kim Y. S. , Kim B. Y. , Kwon S. W. . ( 2010; ). Acinetobacter brisouii sp. nov., isolated from a wetland in Korea. . J Microbiol 48:, 36–39. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bochner B. R. , Gadzinski P. , Panomitros E. . ( 2001; ). Phenotype microarrays for high-throughput phenotypic testing and assay of gene function. . Genome Res 11:, 1246–1255. [CrossRef] [PubMed]
    [Google Scholar]
  5. Bouvet P. J. M. , Grimont P. A. D. . ( 1986; ). Taxonomy of the genus Acinetobacter with the recognition of Acinetobacter baumannii sp. nov., Acinetobacter haemolyticus sp. nov., Acinetobacter johnsonii sp. nov., and Acinetobacter junii sp. nov. and emended descriptions of Acinetobacter calcoaceticus and Acinetobacter lwofii . . Int J Syst Bacteriol 36:, 228–240. [CrossRef]
    [Google Scholar]
  6. Brandenburg A. , Dell’Olivo A. , Bshary R. , Kuhlemeier C. . ( 2009; ). The sweetest thing: advances in nectar research. . Curr Opin Plant Biol 12:, 486–490. [CrossRef] [PubMed]
    [Google Scholar]
  7. Canto A. , Herrera C. M. , Medrano M. , Pérez R. , García I. M. . ( 2008; ). Pollinator foraging modifies nectar sugar composition in Helleborus foetidus (Ranunculaceae):An experimental test. . Am J Bot 95:, 315–320. [CrossRef] [PubMed]
    [Google Scholar]
  8. Cappuccino J. G. , Sherman N. . ( 2002; ). Microbiology: a Laboratory Manual, , 6th edn.. Menlo Park, CA:: Pearson Education/Benjamin Cummings;.
    [Google Scholar]
  9. Carr E. L. , Kämpfer P. , Patel B. K. , Gürtler V. , Seviour R. J. . ( 2003; ). Seven novel species of Acinetobacter isolated from activated sludge. . Int J Syst Evol Microbiol 53:, 953–963. [CrossRef] [PubMed]
    [Google Scholar]
  10. Castresana J. . ( 2000; ). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. . Mol Biol Evol 17:, 540–552. [CrossRef] [PubMed]
    [Google Scholar]
  11. Chenna R. , Sugawara H. , Koike T. , Lopez R. , Gibson T. J. , Higgins D. G. , Thompson J. D. . ( 2003; ). Multiple sequence alignment with the Clustal series of programs. . Nucleic Acids Res 31:, 3497–3500. [CrossRef] [PubMed]
    [Google Scholar]
  12. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  13. Cleenwerck I. , Vandemeulebroecke K. , Janssens D. , Swings J. . ( 2002; ). Re-examination of the genus Acetobacter, with descriptions of Acetobacter cerevisiae sp. nov. and Acetobacter malorum sp. nov.. Int J Syst Evol Microbiol 52:, 1551–1558. [CrossRef] [PubMed]
    [Google Scholar]
  14. Cruze J. A. , Singer J. T. , Finnerty W. R. . ( 1979; ). Conditions for quantitative transformation in Acinetobacter calcoaceticus . . Curr Microbiol 3:, 129–132. [CrossRef]
    [Google Scholar]
  15. de Vega C. , Herrera C. M. , Johnson S. D. . ( 2009; ). Yeasts in floral nectar of some South African plants: quantification and associations with pollinator type and sugar concentration. . S Afr J Bot 75:, 798–806. [CrossRef]
    [Google Scholar]
  16. Doughari H. J. , Ndakidemi P. A. , Human I. S. , Benade S. . ( 2011; ). The ecology, biology and pathogenesis of Acinetobacter spp.: an overview. . Microbes Environ 26:, 101–112. [CrossRef] [PubMed]
    [Google Scholar]
  17. Ezaki T. , Hashimoto T. , Yabuuchi E. . ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridisation in microdilution wells as an alternative to membrane filter hybridisation in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  18. Fridman S. , Izhaki I. , Gerchman Y. , Halpern M. . ( 2012; ). Bacterial communities in floral nectar. . Environ Microbiol Rep 4:, 97–104. [CrossRef]
    [Google Scholar]
  19. Goris J. , Suzuki K. , De Vos P. , Nakase T. , Kersters K. . ( 1998; ). Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  20. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  21. Heil M. . ( 2011; ). Nectar: generation, regulation and ecological functions. . Trends Plant Sci 16:, 191–200. [CrossRef] [PubMed]
    [Google Scholar]
  22. Herrera C. M. , Pozo M. I. . ( 2010; ). Nectar yeasts warm the flowers of a winter-blooming plant. . Proc Biol Sci 277:, 1827–1834. [CrossRef] [PubMed]
    [Google Scholar]
  23. Herrera C. M. , García I. M. , Pérez R. . ( 2008; ). Invisible floral larcenies: microbial communities degrade floral nectar of bumble bee-pollinated plants. . Ecology 89:, 2369–2376. [CrossRef] [PubMed]
    [Google Scholar]
  24. Herrera C. M. , Canto A. , Pozo M. I. , Bazaga P. . ( 2010; ). Inhospitable sweetness: nectar filtering of pollinator-borne inocula leads to impoverished, phylogenetically clustered yeast communities. . Proc Biol Sci 277:, 747–754. [CrossRef] [PubMed]
    [Google Scholar]
  25. Hugh R. , Leifson E. . ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. . J Bacteriol 66:, 24–26.[PubMed]
    [Google Scholar]
  26. Kang Y. S. , Jung J. , Jeon C. O. , Park W. . ( 2011; ). Acinetobacter oleivorans sp. nov. is capable of adhering to and growing on diesel-oil. . J Microbiol 49:, 29–34. [CrossRef] [PubMed]
    [Google Scholar]
  27. Khamis A. , Raoult D. , La Scola B. . ( 2004; ). rpoB gene sequencing for identification of Corynebacterium species. . J Clin Microbiol 42:, 3925–3931. [CrossRef] [PubMed]
    [Google Scholar]
  28. Kim D. , Baik K. S. , Kim M. S. , Park S. C. , Kim S. S. , Rhee M. S. , Kwak Y. S. , Seong C. N. . ( 2008; ). Acinetobacter soli sp. nov., isolated from forest soil. . J Microbiol 46:, 396–401. [CrossRef] [PubMed]
    [Google Scholar]
  29. La Scola B. , Gundi V. A. , Khamis A. , Raoult D. . ( 2006; ). Sequencing of the rpoB gene and flanking spacers for molecular identification of Acinetobacter species. . J Clin Microbiol 44:, 827–832. [CrossRef] [PubMed]
    [Google Scholar]
  30. Malhotra J. , Anand S. , Jindal S. , Rajagopal R. , Lal R. . ( 2012; ). Acinetobacter indicus sp. nov., isolated from a hexachlorocyclohexane dump site. . Int J Syst Evol Microbiol 62:, 2883–2890. [CrossRef] [PubMed]
    [Google Scholar]
  31. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromathography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  32. Nemec A. , Dijkshoorn L. , Cleenwerck I. , De Baere T. , Janssens D. , Van Der Reijden T. J. , Jezek P. , Vaneechoutte M. . ( 2003; ). Acinetobacter parvus sp. nov., a small-colony-forming species isolated from human clinical specimens. . Int J Syst Evol Microbiol 53:, 1563–1567. [CrossRef] [PubMed]
    [Google Scholar]
  33. Nemec A. , Musílek M. , Maixnerová M. , De Baere T. , van der Reijden T. J. K. , Vaneechoutte M. , Dijkshoorn L. . ( 2009; ). Acinetobacter beijerinckii sp. nov. and Acinetobacter gyllenbergii sp. nov., haemolytic organisms isolated from humans. . Int J Syst Evol Microbiol 59:, 118–124. [CrossRef] [PubMed]
    [Google Scholar]
  34. Nishimura Y. , Ino T. , Iizuka H. . ( 1988; ). Acinetobacter radioresistens sp. nov. isolated from cotton and soil. . Int J Syst Bacteriol 38:, 209–211. [CrossRef]
    [Google Scholar]
  35. Peay K. G. , Belisle M. , Fukami T. . ( 2012; ). Phylogenetic relatedness predicts priority effects in nectar yeast communities. . Proc Biol Sci 279:, 749–758. [CrossRef] [PubMed]
    [Google Scholar]
  36. Pozo M. I. , Lachance M.-A. , Herrera C. M. . ( 2012; ). Nectar yeasts of two southern Spanish plants: the roles of immigration and physiological traits in community assembly. . FEMS Microbiol Ecol 80:, 281–293. [CrossRef] [PubMed]
    [Google Scholar]
  37. Sand M. , de Berardinis V. , Mingote A. , Santos H. , Göttig S. , Müller V. , Averhoff B. . ( 2011; ). Salt adaptation in Acinetobacter baylyi: identification and characterization of a secondary glycine betaine transporter. . Arch Microbiol 193:, 723–730. [CrossRef] [PubMed]
    [Google Scholar]
  38. Skerman V. B. D. . ( 1959; ). A Guide to the Identification of the Genera of Bacteria. Baltimore, MA:: The Williams & Wilkins Co;.
    [Google Scholar]
  39. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  40. Towner K. . ( 2006; ). The genus Acinetobacter . . In The Prokaryotes (Proteobacteria, Gamma Subclass) , , 3rd edn., vol. 6, pp. 746–758. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer-Verlag;.[CrossRef]
    [Google Scholar]
  41. Vaneechoutte M. , Nemec A. , Musílek M. , van der Reijden T. J. , van den Barselaar M. , Tjernberg I. , Calame W. , Fani R. , De Baere T. , Dijkshoorn L. . ( 2009; ). Description of Acinetobacter venetianus ex Di Cello et al. 1997 sp. nov.. Int J Syst Evol Microbiol 59:, 1376–1381. [CrossRef] [PubMed]
    [Google Scholar]
  42. Vaz-Moreira I. , Novo A. , Hantsis-Zacharov E. , Lopes A. R. , Gomila M. , Nunes O. C. , Manaia C. M. , Halpern M. . ( 2011; ). Acinetobacter rudis sp. nov., isolated from raw milk and raw wastewater. . Int J Syst Evol Microbiol 61:, 2837–2843. [CrossRef] [PubMed]
    [Google Scholar]
  43. Wilson K. . ( 1987; ). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 241–245. Edited by Ausubel F. M. , Brent R. , Kingston R. E. , Moore D. D. , Seidman J. G. , Smith J. A. , Struhl K. . . New York:: Green Publishing and Wiley-Interscience;.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043489-0
Loading
/content/journal/ijsem/10.1099/ijs.0.043489-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error