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Abstract

A carotenoid-containing chemoheterotrophic bacterium, designated IMCC1507, was isolated from a tidal flat sediment of the Yellow Sea, Korea. Strain IMCC1507 was Gram-negative, yellow, obligately aerobic, non-motile and flexirubin-negative. 16S rRNA gene sequence analysis indicated that strain IMCC1507 belonged to the genus in the family and exhibited 96.1–97.3 % 16S rRNA sequence similarity with the type strains of described species of the genus . DNA–DNA relatedness between strain IMCC1507 and KCCM 42118 ranged from 3.5±2.2 % to 11.2±2.4 %, indicating that strain IMCC1507 represented a novel genomic species in the genus . Chemotaxonomic characteristics of the isolate, i.e. the DNA G+C content (31.4 mol%), iso-C 3-OH, iso-C, iso-C G and anteiso-C as the major fatty acids, MK-6 as the predominant menaquinone and phosphatidylethanolamine as the major polar lipid, were consistent with its assignment to the genus . However, several phenotypic characteristics, including hydrolysis of macromolecules, enzyme activities and carbon source oxidation, differentiated strain IMCC1507 from members of the genus. Data from this study indicate that strain IMCC1507 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is IMCC1507 ( = KACC 14312  = NBRC 107589).

Funding
This study was supported by the:
  • , National Institute of Biological Resources
  • , NIBR
  • , Inha University
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2013-03-01
2020-10-21
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References

  1. Bernardet J.-F., Nakagawa Y. ( 2006 ). An introduction to the family Flavobacteriaceae . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 455480. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  2. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. ( 1996 ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46, 128148. [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 10491070. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  5. Cho J.-C., Giovannoni S. J. ( 2003 ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the alpha-Proteobacteria . . Int J Syst Evol Microbiol 53, 10311036. [CrossRef] [PubMed]
    [Google Scholar]
  6. Choi D. H., Cho B. C. ( 2006 ). Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. . Int J Syst Evol Microbiol 56, 771776. [CrossRef] [PubMed]
    [Google Scholar]
  7. Choo Y.-J., Lee K., Song J., Cho J.-C. ( 2007 ). Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. . Int J Syst Evol Microbiol 57, 532537. [CrossRef] [PubMed]
    [Google Scholar]
  8. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y.-W. ( 2007 ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57, 22592261. [CrossRef] [PubMed]
    [Google Scholar]
  9. Collins M. D. ( 1985 ). Analysis of isoprenoid quinones. . Methods Microbiol 18, 329366. [CrossRef]
    [Google Scholar]
  10. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  11. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  12. Kim K.-Y., Park S.-J., Hahm Y.-T., Cha C.-J. ( 2011 ). Marinitalea sucinacia gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. . FEMS Microbiol Lett 314, 8994. [CrossRef] [PubMed]
    [Google Scholar]
  13. Komagata K., Suzuki K. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [CrossRef]
    [Google Scholar]
  14. Lee S.-Y., Lee M.-H., Oh T.-K., Yoon J.-H. ( 2012 ). Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006. . Int J Syst Evol Microbiol 62, 420424. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [CrossRef]
    [Google Scholar]
  18. Park S., Kang S.-J., Oh T.-K., Yoon J.-H. ( 2010 ). Lutibacter maritimus sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 60, 610614. [CrossRef] [PubMed]
    [Google Scholar]
  19. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. ( 2007 ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35, 71887196. [CrossRef] [PubMed]
    [Google Scholar]
  20. Reichenbach H. ( 1992 ). The order Cytophagales . . In The Prokaryotes, , 2nd edn., vol. 4, pp. 36313675. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhart P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
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