1887

Abstract

A carotenoid-containing chemoheterotrophic bacterium, designated IMCC1507, was isolated from a tidal flat sediment of the Yellow Sea, Korea. Strain IMCC1507 was Gram-negative, yellow, obligately aerobic, non-motile and flexirubin-negative. 16S rRNA gene sequence analysis indicated that strain IMCC1507 belonged to the genus in the family and exhibited 96.1–97.3 % 16S rRNA sequence similarity with the type strains of described species of the genus . DNA–DNA relatedness between strain IMCC1507 and KCCM 42118 ranged from 3.5±2.2 % to 11.2±2.4 %, indicating that strain IMCC1507 represented a novel genomic species in the genus . Chemotaxonomic characteristics of the isolate, i.e. the DNA G+C content (31.4 mol%), iso-C 3-OH, iso-C, iso-C G and anteiso-C as the major fatty acids, MK-6 as the predominant menaquinone and phosphatidylethanolamine as the major polar lipid, were consistent with its assignment to the genus . However, several phenotypic characteristics, including hydrolysis of macromolecules, enzyme activities and carbon source oxidation, differentiated strain IMCC1507 from members of the genus. Data from this study indicate that strain IMCC1507 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is IMCC1507 ( = KACC 14312  = NBRC 107589).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043471-0
2013-03-01
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/946.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043471-0&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F. , Nakagawa Y. . ( 2006; ). An introduction to the family Flavobacteriaceae . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 455–480. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K.-H. , Stackebrandt E. . . New York:: Springer;.
    [Google Scholar]
  2. Bernardet J.-F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46:, 128–148. [CrossRef]
    [Google Scholar]
  3. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  4. Bowman J. P. . ( 2000; ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  5. Cho J.-C. , Giovannoni S. J. . ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the alpha-Proteobacteria . . Int J Syst Evol Microbiol 53:, 1031–1036. [CrossRef] [PubMed]
    [Google Scholar]
  6. Choi D. H. , Cho B. C. . ( 2006; ). Lutibacter litoralis gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. . Int J Syst Evol Microbiol 56:, 771–776. [CrossRef] [PubMed]
    [Google Scholar]
  7. Choo Y.-J. , Lee K. , Song J. , Cho J.-C. . ( 2007; ). Puniceicoccus vermicola gen. nov., sp. nov., a novel marine bacterium, and description of Puniceicoccaceae fam. nov., Puniceicoccales ord. nov., Opitutaceae fam. nov., Opitutales ord. nov. and Opitutae classis nov. in the phylum ‘Verrucomicrobia’. . Int J Syst Evol Microbiol 57:, 532–537. [CrossRef] [PubMed]
    [Google Scholar]
  8. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y.-W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  9. Collins M. D. . ( 1985; ). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  10. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  11. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  12. Kim K.-Y. , Park S.-J. , Hahm Y.-T. , Cha C.-J. . ( 2011; ). Marinitalea sucinacia gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from tidal flat sediment. . FEMS Microbiol Lett 314:, 89–94. [CrossRef] [PubMed]
    [Google Scholar]
  13. Komagata K. , Suzuki K. . ( 1987; ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  14. Lee S.-Y. , Lee M.-H. , Oh T.-K. , Yoon J.-H. . ( 2012; ). Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006. . Int J Syst Evol Microbiol 62:, 420–424. [CrossRef] [PubMed]
    [Google Scholar]
  15. Ludwig W. , Strunk O. , Westram R. , Richter L. , Meier H. , Yadhukumar , Buchner A. , Lai T. , Steppi S. . & other authors ( 2004; ). arb: a software environment for sequence data. . Nucleic Acids Res 32:, 1363–1371. [CrossRef] [PubMed]
    [Google Scholar]
  16. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E. , O'Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Park S. , Kang S.-J. , Oh T.-K. , Yoon J.-H. . ( 2010; ). Lutibacter maritimus sp. nov., isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 60:, 610–614. [CrossRef] [PubMed]
    [Google Scholar]
  19. Pruesse E. , Quast C. , Knittel K. , Fuchs B. M. , Ludwig W. , Peplies J. , Glöckner F. O. . ( 2007; ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. . Nucleic Acids Res 35:, 7188–7196. [CrossRef] [PubMed]
    [Google Scholar]
  20. Reichenbach H. . ( 1992; ). The order Cytophagales . . In The Prokaryotes, , 2nd edn., vol. 4, pp. 3631–3675. Edited by Balows A. , Trüper H. G. , Dworkin M. , Harder W. , Schleifer K. H. . . New York:: Springer;.[CrossRef]
    [Google Scholar]
  21. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  22. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhart P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043471-0
Loading
/content/journal/ijsem/10.1099/ijs.0.043471-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error