1887

Abstract

A bacterial strain, designated HOR6-4, was isolated from the epidermal tissue of a root of a orchid. Strain HOR6-4 was characterized using a polyphasic approach. The cells were aerobic, Gram-stain-positive, non-motile coccoids. Phylogenetic analysis of its 16S rRNA gene sequence revealed a clear affiliation with the family . Strain HOR6-4 showed relatively low 16S rRNA gene sequence similarity (below 95.9 %) to type strains of species with validly published names, showing the highest sequence similarities to the type strains of (95.8 %) and (95.5 %). The DNA G+C content of strain HOR6-4 was 64.7 mol%. Strain HOR6-4 had anteiso-C (19.3 %), 10-methyl C (tuberculostearic acid; 13.5 %) and 10-methyl C (11.7 %) as the major fatty acids and contained MK-8(H) and MK-8(H) as the predominant quinones. Polar lipids were diphosphatidylglycerol, phosphatidylinositol, three aminophospholipids, two phospholipids and an aminolipid. The peptidoglycan type was A4α, with an -Lys–-Thr–-Glu interpeptide bridge with a glycine residue bound to the alpha-carboxyl group of -Glu in position 2 of the peptide subunit. Mycolic acids were not present. On the basis of comparative analysis of phenotypic and phylogenetic traits, strain HOR6-4 is considered to represent a novel species in a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is HOR6-4 ( = KACC 12637  = DSM 19560).

Funding
This study was supported by the:
  • , National Academy of Agricultural Science, Rural Development Administration, Republic of Korea , (Award PJ008666)
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2013-04-01
2020-09-25
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