1887

Abstract

A novel aerobic bacterium, designated strain PIII.02, was isolated from a Mediterranean sponge () collected off the Israeli coast near Sdot Yam. The non-motile cells were Gram-staining-negative, oxidase-positive and catalase-positive. The orange pigment of colonies growing on marine agar was neither diffusible nor flexirubin-like. Strain PIII.02 grew at 15–35 °C, at pH 6.0–9.0, with 2.0–7.0 % (w/v) NaCl, and with 1.0-8.0 % (w/v) sea salts. The predominant fatty acids were iso-C, iso-C H, iso-C, C, anteiso-C and Cω7. The major respiratory quinone was MK-7. The genomic DNA G+C content of the novel strain was 38.1 mol%. Results from 16S rRNA gene sequence analysis indicated that strain PIII.02 was distantly related to established members of the phylum . The established species found to be most closely related to the novel strain was NCIMB 1402 (87.6 % 16S rRNA gene sequence similarity). Based on the phenotypic and chemotaxonomic data and the results of the phylogenetic analyses, strain PIII.02 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is PIII.02 ( = ATCC BAA-2393  = LMG 26723).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043398-0
2013-03-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/3/939.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043398-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Bernardet J.-F. , Nakagawa Y. , Holmes B. . Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  3. Castresana J. . ( 2000; ). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. . Mol Biol Evol 17:, 540–552. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cole J. R. , Wang Q. , Cardenas E. , Fish J. , Chai B. , Farris R. J. , Kulam-Syed-Mohideen A. S. , McGarrell D. M. , Marsh T. . & other authors ( 2009; ). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37: (Database issue), D141–D145. [CrossRef] [PubMed]
    [Google Scholar]
  5. Dereeper A. , Guignon V. , Blanc G. , Audic S. , Buffet S. , Chevenet F. , Dufayard J. F. , Guindon S. , Lefort V. . & other authors ( 2008; ). Phylogeny.fr: robust phylogenetic analysis for the non-specialist. . Nucleic Acids Res 36: (Web Server issue), W465–W469. [CrossRef] [PubMed]
    [Google Scholar]
  6. Guindon S. , Dufayard J. F. , Lefort V. , Anisimova M. , Hordijk W. , Gascuel O. . ( 2010; ). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. . Syst Biol 59:, 307–321. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kelman D. , Kashman Y. , Rosenberg E. , Ilan M. , Ifrach I. , Loya Y. . ( 2001; ). Antimicrobial activity of the reef sponge Amphimedon viridis from the Red Sea: evidence for selective toxicity. . Aquat Microb Ecol 24:, 9–16. [CrossRef]
    [Google Scholar]
  8. Kim O. S. , Cho Y. J. , Lee K. , Yoon S. H. , Kim M. , Na H. , Park S. C. , Jeon Y. S. , Lee J. H. . & other authors ( 2012; ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef] [PubMed]
    [Google Scholar]
  9. Lau S. C. K. , Tsoi M. M. Y. , Li X. , Plakhotnikova I. , Dobretsov S. , Wu M. , Wong P. K. , Pawlik J. R. , Qian P. Y. . ( 2006; ). Description of Fabibacter halotolerans gen. nov., sp. nov. and Roseivirga spongicola sp. nov., and reclassification of (Marinicola) seohaensis as Roseivirga seohaensis comb. nov.. Int J Syst Evol Microbiol 56:, 1059–1065. [CrossRef] [PubMed]
    [Google Scholar]
  10. Marchesi J. R. , Sato T. , Weightman A. J. , Martin T. A. , Fry J. C. , Hiom S. J. , Dymock D. , Wade W. G. . ( 1998; ). Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. . Appl Environ Microbiol 64:, 795–799.[PubMed]
    [Google Scholar]
  11. MIDI ( 1999; ). Sherlock Microbial Identification System Operating Manual, version 3.0. . Newark, DE:: MIDI Inc.;
  12. Muramatsu Y. , Takahashi M. , Kaneyasu M. , Iino T. , Suzuki K. I. , Nakagawa Y. . ( 2010; ). Persicobacter psychrovividus sp. nov., isolated from shellfish, and emended descriptions of the genus Persicobacter and Persicobacter diffluens . . Int J Syst Evol Microbiol 60:, 1735–1739. [CrossRef] [PubMed]
    [Google Scholar]
  13. Nawrocki E. P. , Kolbe D. L. , Eddy S. R. . ( 2009; ). Infernal 1.0: inference of RNA alignments. . Bioinformatics 25:, 1335–1337. [CrossRef] [PubMed]
    [Google Scholar]
  14. Nedashkovskaya O. I. , Kim S. B. , Lee D. H. , Lysenko A. M. , Shevchenko L. S. , Frolova G. M. , Mikhailov V. V. , Lee K. H. , Bae K. S. . ( 2005a; ). Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’, isolated from the green alga Ulva fenestrata . . Int J Syst Evol Microbiol 55:, 231–234. [CrossRef] [PubMed]
    [Google Scholar]
  15. Nedashkovskaya O. I. , Kim S. B. , Lysenko A. M. , Park M. S. , Mikhailov V. V. , Bae K. S. , Park H. Y. . ( 2005b; ). Roseivirga echinicomitans sp. nov., a novel bacterium isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Roseivirga . . Int J Syst Evol Microbiol 55:, 1797–1800. [CrossRef] [PubMed]
    [Google Scholar]
  16. Pimentel-Elardo S. M. , Scheuermayer M. , Kozytska S. , Hentschel U. . ( 2009; ). Streptomyces axinellae sp. nov., isolated from the Mediterranean sponge Axinella polypoides (Porifera). . Int J Syst Evol Microbiol 59:, 1433–1437. [CrossRef] [PubMed]
    [Google Scholar]
  17. Poli A. , Romano I. , Cordella P. , Orlando P. , Nicolaus B. , Ceschi Berrini C. . ( 2009; ). Anoxybacillus thermarum sp. nov., a novel thermophilic bacterium isolated from thermal mud in Euganean hot springs, Abano Terme, Italy. . Extremophiles 13:, 867–874. [CrossRef] [PubMed]
    [Google Scholar]
  18. Posada D. , Crandall K. A. . ( 1998; ). MODELTEST: testing the model of DNA substitution. . Bioinformatics 14:, 817–818. [CrossRef] [PubMed]
    [Google Scholar]
  19. Romano I. , Nicolaus B. , Lama L. , Trabasso D. , Caracciolo G. , Gambacorta A. . ( 2001; ). Accumulation of osmoprotectants and lipid pattern modulation in response to growth conditions by Halomonas pantelleriense . . Syst Appl Microbiol 24:, 342–352. [CrossRef] [PubMed]
    [Google Scholar]
  20. Scheuermayer M. , Gulder T. A. M. , Bringmann G. , Hentschel U. . ( 2006; ). Rubritalea marina gen. nov., sp. nov., a marine representative of the phylum ‘Verrucomicrobia’, isolated from a sponge (Porifera). . Int J Syst Evol Microbiol 56:, 2119–2124. [CrossRef] [PubMed]
    [Google Scholar]
  21. Schmitt S. , Tsai P. , Bell J. , Fromont J. , Ilan M. , Lindquist N. , Perez T. , Rodrigo A. , Schupp P. J. . & other authors ( 2012; ). Assessing the complex sponge microbiota: core, variable and species-specific bacterial communities in marine sponges. . ISME J 6:, 564–576. [CrossRef] [PubMed]
    [Google Scholar]
  22. Simister R. L. , Deines P. , Botté E. S. , Webster N. S. , Taylor M. W. . ( 2012; ). Sponge-specific clusters revisited: a comprehensive phylogeny of sponge-associated microorganisms. . Environ Microbiol 14:, 517–524. [CrossRef] [PubMed]
    [Google Scholar]
  23. Swofford D. L. . ( 2003; ). paup*: Phylogenetic analysis using parsimony (and other methods), version 4. . Sunderland, MA:: Sinauer Associates;.
  24. Tamura K. , Peterson D. , Peterson N. , Stecher G. , Nei M. , Kumar S. . ( 2011; ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef] [PubMed]
    [Google Scholar]
  25. Taylor M. W. , Radax R. , Steger D. , Wagner M. . ( 2007; ). Sponge-associated microorganisms: evolution, ecology, and biotechnological potential. . Microbiol Mol Biol Rev 71:, 295–347. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043398-0
Loading
/content/journal/ijsem/10.1099/ijs.0.043398-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error