A novel actinobacterium strain, 2614A723T, was isolated from rhizosphere soil of mangrove plant Acanthus ilicifolius collected at Touyuan, Wenchang, Hainan province, China. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 2614A723T formed a distinct phyletic line in the genus Actinoallomurus, the 16S rRNA gene tree sharing similarities of 98.35 %, 98.07 % and 97.86 % with Actinoallomurus spadix NBRC 14099T, Actinoallomurus purpureus TTN02-30T and Actinoallomurus luridus TT02-15T, respectively. Strain 2614A723T contained lysine and meso-diaminopimelic acid in the cell wall peptidoglycan and madurose, galactose and xylose in the whole-cell sugars. The predominant menaquinones were MK-9(H4) and MK-9(H6). The major polar phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The predominant fatty acids were iso-C16 : 0 and anteiso-C17 : 0. These chemotaxonomic data confirmed the affiliation of strain 2614A723T to the genus Actinoallomurus. It is apparent from the combined phenotypic data, biochemical tests and DNA–DNA hybridization values that strain 2614A723T should be classified in the genus Actinoallomurus as a representative of a novel species. The name Actinoallomurus acanthiterrae sp. nov. is proposed with strain 2614A723T ( = CCTCC AA 2012001T = DSM 45727T) as the type strain.
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 31170467)
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