1887

Abstract

Two bacterial strains, VI.14 and VIII.04, were isolated from the Mediterranean sponge collected off the Israeli coast near Sdot Yam. The non-motile, aerobic, Gram-negative isolates were oxidase-negative and catalase-positive, and formed golden-brown colonies on marine agar 2216. The pigment was neither diffusible nor flexirubin-like. Strain VIII.04 grew at 15–37 °C, at pH 6.0–9.0, in the presence of 20–50 g NaCl l and 20–80 g sea salts l, The spectrum was narrower for strain VI.14, with growth at pH 7.0–8.0. and in the presence of 30–50 g NaCl l and 30–70 g sea salts l. The predominant fatty acid (>50 %) in both strains was iso-C, and the major respiratory quinone was MK-6. The DNA G+C content was 30.7 and 31.1 mol% for VIII.04 and VI.14, respectively. Results from 16S rRNA sequence similarity and phylogenetic analyses indicated that both strains are closely related to members of the family within the phylum , with as much as 91.7 % 16S rRNA sequence similarity. On the basis of data from the polyphasic analysis, we suggest that the strains represent a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. Strain VIII.04 ( = ATCC BAA-2394 = LMG 26721) is the type strain of .

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2013-03-01
2020-01-27
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