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Volume 63, Issue Pt_3

gen. nov., sp. nov., a marine bacterium isolated from the Mediterranean sponge Free

Abstract

Two bacterial strains, VI.14 and VIII.04, were isolated from the Mediterranean sponge collected off the Israeli coast near Sdot Yam. The non-motile, aerobic, Gram-negative isolates were oxidase-negative and catalase-positive, and formed golden-brown colonies on marine agar 2216. The pigment was neither diffusible nor flexirubin-like. Strain VIII.04 grew at 15–37 °C, at pH 6.0–9.0, in the presence of 20–50 g NaCl l and 20–80 g sea salts l, The spectrum was narrower for strain VI.14, with growth at pH 7.0–8.0. and in the presence of 30–50 g NaCl l and 30–70 g sea salts l. The predominant fatty acid (>50 %) in both strains was iso-C, and the major respiratory quinone was MK-6. The DNA G+C content was 30.7 and 31.1 mol% for VIII.04 and VI.14, respectively. Results from 16S rRNA sequence similarity and phylogenetic analyses indicated that both strains are closely related to members of the family within the phylum , with as much as 91.7 % 16S rRNA sequence similarity. On the basis of data from the polyphasic analysis, we suggest that the strains represent a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. Strain VIII.04 ( = ATCC BAA-2394 = LMG 26721) is the type strain of .

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Funding
This study was supported by the:
  • European Union (EU) Research Training Network (RTN) (Award MRTN-CT- 2004-512301 and 339 FP6, BIOCAPI AL)
© 2013 IUMS
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2013-03-01
2024-04-24
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Aureivirga marina gen. nov., sp. nov., a marine bacterium isolated from the Mediterranean sponge Axinella verrucosa
Int J Syst Evol Microbiol 63, 1089 (2013); https://doi.org/10.1099/ijs.0.043257-0
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