1887

Abstract

A heterotrophic marine bacterium, designated strain KTSW-6, was isolated from the reef-building coral in Kenting, Taiwan. Cells of strain KTSW-6 were Gram-stain-negative, facultatively anaerobic, halophilic, non-motile rods surrounded by a thick glycocalyx-like coat and forming creamy white colonies. Growth occurred at 15–37 °C (optimum, 25–30 °C), at pH 7.0–9.0 (optimum, pH 7.5–8.0) and with 0.5–7 % NaCl (optimum, 3–4 %). Polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an uncharacterized aminophospholipid and three uncharacterized phospholipids (PL1–PL3). The respiratory quinones of strain KTSW-6 were Q-8 (62 %) and Q-7 (38 %). Major cellular fatty acids were summed feature 3 (Cω7 and/or Cω6, 29.6 %), Cω7 (27.6 %) and C (19.5 %). The major cellular hydroxy fatty acid was C 3-OH. The DNA GC content of strain KTSW-6 was 48.6 mol%. 16S rRNA gene sequence analysis indicated that strain KTSW-6 belongs to the family of the order , class Strain KTSW-6 shared 92.7 % 16S rRNA gene sequence similarity with MED92 and 92.0 % with NAG-2N-126. On the basis of the genotypic and phenotypic data, strain KTSW-6 represents a novel species of a new genus of the , for which the name gen. nov., sp. nov. is proposed. The type strain of is KTSW-6 ( = BCRC 80176 = LMG 25553).

Funding
This study was supported by the:
  • , Academia Sinica , (Award 20082010)
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2013-03-01
2020-07-12
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References

  1. Anzai Y., Kudo Y., Oyaizu H. ( 1997 ). The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera. . Int J Syst Bacteriol 47, 249251.[PubMed] [CrossRef]
    [Google Scholar]
  2. Arahal D. R., Lekunberri I., González J. M., Pascual J., Pujalte M. J., Pedrós-Alió C., Pinhassi J. ( 2007 ). Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium. . Int J Syst Evol Microbiol 57, 10001006.[PubMed] [CrossRef]
    [Google Scholar]
  3. Beveridge T. J., Lawrence J. R., Murray R. G. E. ( 2007 ). Sampling and staining for light microscopy. . In Methods for General and Molecular Bacteriology, , 3rd edn., pp. 1933. Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 18611868.[PubMed]
    [Google Scholar]
  5. Breznak J. A., Costilow R. N. ( 2007 ). Physicochemical factors in growth. . In Methods for General and Molecular Bacteriology, , 3rd edn., pp. 309329. Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  6. Chen W. M., Laevens S., Lee T. M., Coenye T., De Vos P., Mergeay M., Vandamme P. ( 2001 ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51, 17291735.[PubMed] [CrossRef]
    [Google Scholar]
  7. Choi D. H., Cho B. C. ( 2006 ). Shimia marina gen. nov., sp. nov., a novel bacterium of the Roseobacter clade isolated from biofilm in a coastal fish farm. . Int J Syst Evol Microbiol 56, 18691873.[PubMed] [CrossRef]
    [Google Scholar]
  8. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
    [Google Scholar]
  9. Embley T. M., Wait R. ( 1994 ). Structural lipids of eubacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 121161. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  10. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376.[PubMed] [CrossRef]
    [Google Scholar]
  11. Felsenstein J. ( 1993 ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Hall T. A. ( 1999 ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41, 9598.
    [Google Scholar]
  13. Hedlund B. P., Geiselbrecht A. D., Bair T. J., Staley J. T. ( 1999 ). Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov.. Appl Environ Microbiol 65, 251259.[PubMed]
    [Google Scholar]
  14. Hosoya S., Adachi K., Kasai H. ( 2009 ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59, 686690.[PubMed] [CrossRef]
    [Google Scholar]
  15. Hsu S. C., Lockwood J. L. ( 1975 ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29, 422426.[PubMed]
    [Google Scholar]
  16. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kimura M. ( 1983 ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  18. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [CrossRef]
    [Google Scholar]
  19. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenegger M., Neumaier J., Bachleitner M., Schleifer K. H. ( 1998 ). Bacterial phylogeny based on comparative sequence analysis. . Electrophoresis 19, 554568.[PubMed] [CrossRef]
    [Google Scholar]
  20. Lyman J., Fleming R. H. ( 1940 ). Composition of sea water. . J Mar Res 3, 134146.
    [Google Scholar]
  21. Maidak B. L., Cole J. R., Lilburn T. G., Parker C. T. Jr, Saxman P. R., Farris R. J., Garrity G. M., Olsen G. J., Schmidt T. M., Tiedje J. M. ( 2001 ). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29, 173174.[PubMed] [CrossRef]
    [Google Scholar]
  22. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the GC content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  23. Miyazaki M., Nogi Y., Fujiwara Y., Kawato M., Kubokawa K., Horikoshi K. ( 2008 ). Neptunomonas japonica sp. nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan. . Int J Syst Evol Microbiol 58, 866871.[PubMed] [CrossRef]
    [Google Scholar]
  24. Powers E. M. ( 1995 ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61, 37563758.[PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739.[PubMed] [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882.[PubMed] [CrossRef]
    [Google Scholar]
  28. Tindall B. J., Sikorski J., Smibert R. A., Krieg N. R. ( 2007 ). Phenotypic characterization and the principles of comparative systematics. . In Methods for General and Molecular Bacteriology, , 3rd edn., pp. 330393. Edited by Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  29. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991 ). 16S ribosomal DNA amplification for phylogenetic study. . J Bacteriol 173, 697703.[PubMed]
    [Google Scholar]
  30. Yakimov M. M., Golyshin P. N., Lang S., Moore E. R. B., Abraham W.-R., Lünsdorf H., Timmis K. N. ( 1998 ). Alcanivorax borkumensis gen. nov., sp. nov., a new, hydrocarbon-degrading and surfactant-producing marine bacterium. . Int J Syst Bacteriol 48, 339348.[PubMed] [CrossRef]
    [Google Scholar]
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