1887

Abstract

A novel actinobacterial strain was isolated from a seawater sample collected on Mara Island, Jeju, Republic of Korea. Cells of this organism were aerobic, Gram-positive, non-spore-forming, non-motile cocci that occurred singly or in pairs. Colonies were circular, smooth, convex and white–cream in colour. Phylogenetic analyses based on 16S rRNA gene sequences showed that the organism belonged to the family and formed a monophyletic clade between the type strains of (96.8 % similarity) and (95.2 % similarity). The cell-wall peptidoglycan contained -lysine, alanine, aspartic acid, glutamic acid, glycine and serine, indicating that the isolate possessed peptidoglycan type A4α. The whole-cell sugars were galactose, glucose, mannose, xylose, arabinose, ribose and rhamnose. The major menaquinone was MK-8(H). The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and five unknown lipids. The cellular fatty acid profile was represented by large amounts of iso-methyl branched and monounsaturated iso- and anteiso-methyl branched acids, along with the presence of a diagnostic 10-methyl acid. The G+C content of the DNA was 71 mol%. On the basis of data from polyphasic analyses presented here, strain MSW-24 is considered to represent a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is MSW-24 ( = KCTC 19485 = DSM 21415).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.043117-0
2013-06-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/6/1951.html?itemId=/content/journal/ijsem/10.1099/ijs.0.043117-0&mimeType=html&fmt=ahah

References

  1. Ara I., Yamamura H., Tsetseg B., Daram D., Ando K.. ( 2010;). Luteipulveratus mongoliensis gen. nov., sp. nov., an actinobacterial taxon in the family Dermacoccaceae. . Int J Syst Evol Microbiol 60:, 574–579. [CrossRef][PubMed]
    [Google Scholar]
  2. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  3. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Fitch W. M.. ( 1971;). Towards defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef][PubMed]
    [Google Scholar]
  6. Hancock I. C.. ( 1994;). Analysis of cell wall constituents of Gram-positive bacteria. . In Chemical Methods in Prokaryotic Systematics, pp. 63–84. Edited by Goodfellow M., O’Donnell A. G... Chichester:: John Wiley & Sons;.
    [Google Scholar]
  7. Jukes T. H., Cantor C. R.. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro H. N... New York:: Academic Press;.
    [Google Scholar]
  8. Kämpfer P., Martin K., Schäfer J., Schumann P.. ( 2009;). Kytococcus aerolatus sp. nov., isolated from indoor air in a room colonized with moulds. . Syst Appl Microbiol 32:, 301–305. [CrossRef][PubMed]
    [Google Scholar]
  9. Kroppenstedt R. M.. ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  10. Lee S. D.. ( 2007;). Labedella gwakjiensis gen. nov., sp. nov., a novel actinomycete of the family Microbacteriaceae. . Int J Syst Evol Microbiol 57:, 2498–2502. [CrossRef][PubMed]
    [Google Scholar]
  11. Lee S. D., Kang S.-O., Hah Y. C.. ( 2000;). Hongia gen. nov., a new genus of the order Actinomycetales. . Int J Syst Evol Microbiol 50:, 191–199. [CrossRef][PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B.. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., Patel P. V., Alshamaony L., Goodfellow M.. ( 1977;). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol 27:, 104–117. [CrossRef]
    [Google Scholar]
  14. Pathom-aree W., Nogi Y., Sutcliffe I. C., Ward A. C., Horikoshi K., Bull A. T., Goodfellow M.. ( 2006a;). Dermacoccus abyssi sp. nov., a piezotolerant actinomycete isolated from the Mariana Trench. . Int J Syst Evol Microbiol 56:, 1233–1237. [CrossRef][PubMed]
    [Google Scholar]
  15. Pathom-aree W., Nogi Y., Ward A. C., Horikoshi K., Bull A. T., Goodfellow M.. ( 2006b;). Dermacoccus barathri sp. nov. and Dermacoccus profundi sp. nov., novel actinomycetes isolated from deep-sea mud of the Mariana Trench. . Int J Syst Evol Microbiol 56:, 2303–2307. [CrossRef][PubMed]
    [Google Scholar]
  16. Saddler G. S., Tavecchia P., Lociuro S., Zanol M., Colombo E., Selva E.. ( 1991;). Analysis of madurose and other actinomycete whole cell sugars by gas chromatography. . J Microbiol Methods 14:, 185–191. [CrossRef]
    [Google Scholar]
  17. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Stackebrandt E., Schumann P.. ( 2000;). Description of Bogoriellaceae fam. nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov. and emendation of some families of the suborder Micrococcineae. . Int J Syst Evol Microbiol 50:, 1279–1285. [CrossRef][PubMed]
    [Google Scholar]
  19. Stackebrandt E., Koch C., Gvozdiak O., Schumann P.. ( 1995;). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. . Int J Syst Bacteriol 45:, 682–692. [CrossRef][PubMed]
    [Google Scholar]
  20. Sugimoto S., Kato T., Ito M., Sakata N., Tsuchida T., Matsumoto A., Takahashi Y.. ( 2011;). Branchiibius hedensis gen. nov., sp. nov., an actinobacterium isolated from a Japanese codling (Physiculus japonicus). . Int J Syst Evol Microbiol 61:, 1195–1200. [CrossRef][PubMed]
    [Google Scholar]
  21. Tang S.-K., Wu J.-Y., Wang Y., Schumann P., Li W.-J.. ( 2010;). Yimella lutea gen. nov., sp. nov., a novel actinobacterium of the family Dermacoccaceae. . Int J Syst Evol Microbiol 60:, 659–663. [CrossRef][PubMed]
    [Google Scholar]
  22. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.043117-0
Loading
/content/journal/ijsem/10.1099/ijs.0.043117-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error