1887

Abstract

A yellow pigmented, Gram-negative, rod-shaped bacterium designated FNE08-7 was isolated from subsurface water of the north-east basin of the bog lake Grosse Fuchskuhle (Brandenburg, Germany). A first analysis of the nearly full-length 16S rRNA gene sequence analysis including environmental 16S rRNA gene sequences derived from freshwater ecosystems showed that strain FNE08-7 is the first cultured representative, to our knowledge, of the freshwater tribe Novo-A2. Further analysis indicates highest 16S rRNA gene sequence similarities to the type strains of (98.0 %) and (97.4 %) and between 94.0 % and 96.9 % sequence similarity to other members of the genus . Reconstruction of phylogenetic trees showed that strain FNE08-7 formed a distinct cluster with the type strains of and supported by high bootstrap values. DNA–DNA hybridization of strain FNE08-7 with SMCC B0712 and DSM 17507 revealed low similarity values of 18.4 % (reciprocal: 11.4 %) and 23.1 % (reciprocal: 54.2 %), respectively. The predominant fatty acid of the isolate is Cω7 (56.4 %) and two characteristic 2-hydroxy fatty acids, C 2-OH (16.5 %) and C 2-OH (3.3 %) occur. Ubiquinone Q-10 is the major respiratory quinone. The predominant polar lipids are phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine and minor amounts of diphosphatidylglycerol. Spermidine is the predominant polyamine. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicate that strain FNE08-7 represents a novel species of the genus within the . Therefore, we propose the species sp. nov., with FNE08-7 ( = DSM 25065 = CCM 7978 = CCUG 61508) as the type strain.

Funding
This study was supported by the:
  • German Science Foundation (DFG) (Award JG-620/2-1)
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2013-02-01
2021-08-04
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