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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 63, Issue Pt_7

sp. nov., a polysaccharide-degrading bacterium isolated from an Indian mangrove Free

Abstract

A rod-shaped, Gram-negative, non-motile, aerobic and non-endospore forming bacterium, designated strain DD-13, was isolated from the mangrove ecosystem of Goa, India. Strain DD-13 degraded polysaccharides such as agar, alginate, chitin, cellulose, laminarin, pectin, pullulan, starch, carrageenan, xylan and β-glucan. The optimum pH and temperature for growth was 7 and 36 °C, respectively. The strain grew optimally in the presence of 3 % NaCl (w/v). The DNA G+C content was 61.4 mol%. The predominant fatty acid of strain DD-13 was iso-C. Ubiquinone-8 was detected as the major respiratory lipoquinone. Phylogenetic studies based on 16S rRNA gene sequence analysis demonstrated that strain DD-13 formed a coherent cluster with species of the genus . Strain DD-13 exhibited 16S rRNA gene sequence similarity levels of 98.9–97.1 % with IRE-31, JCM 11542, JAMB A3, KCTC 12973 and DSM 6810. However, the level of DNA–DNA relatedness between strain DD-13 and the five type strains of these species of the genus were in the range of 26–33 %. Additionally, strain DD-13 demonstrates several phenotypic differences from these type strains of species of the genus . Thus strain DD-13 represents a novel species of the genus , for which the name sp. nov. is proposed with the type strain DD-13 ( = KCTC 23483 = JCM 17729).

  • Published Online:
Funding
This study was supported by the:
  • Goa University
© 2013 IUMS
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2013-07-01
2024-04-24
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Microbulbifer mangrovi sp. nov., a polysaccharide-degrading bacterium isolated from an Indian mangrove
Int J Syst Evol Microbiol 63, 2532 (2013); https://doi.org/10.1099/ijs.0.042978-0
/content/journal/ijsem/10.1099/ijs.0.042978-0
/content/journal/ijsem/10.1099/ijs.0.042978-0
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