1887

Abstract

A novel type of freshwater bacterium was isolated from the prealpine mesotrophic Starnberger See (Bavaria, southern Germany). Cells of strain 382 were Gram-negative and rod-shaped, motile and creamy-white. The isolate was strictly aerobic, catalase- and oxidase-positive, and grew at pH values of 6–9 (optimum, pH 7) and temperatures of 10–37 °C (optimum, 28 °C). The genomic G+C content of strain 382 was 64.1 mol%. Based on 16S rRNA gene sequence analyses, strain 382 belongs to the family and clusters within the genus . UM 2 and DSM 6014 were the closest relatives, as indicated by the highest 16S rRNA gene sequence similarities (97.1 % and 96.8 %, respectively). DSM 1098 (the type species of the genus ) exhibited 95.3 % sequence similarity. This affiliation of strain 382 to the genus is confirmed by the presence of Q-10 as the major respiratory quinone, two sphingoglycolipids, C 2-OH as the major 2-hydroxy fatty acid and -homospermidine as the major polyamine. The main cellular fatty acids of strain 382 were Cω7 (39 %), Cω7 (21 %), C (10 %) and C 2-OH (10 %). Based on the phylogenetic distance from other species of the genus and its unusually high Cω7 content, strain 382 represents a novel species of the genus , for which the name is proposed. The type strain is 382 ( = DSM 25077  = LMG 26763).

Funding
This study was supported by the:
  • , Deutsche Forschungsgemeinschaft , (Award OV 20/19-1 and OV 20/17-1)
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2013-03-01
2020-11-24
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