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Abstract

A Gram-negative, non-spore-forming, non-flagellated, motile-by-gliding rod, designated SSK2-3, was isolated from the junction between seawater and a freshwater spring at Jeju island, South Korea. Strain SSK2-3 grew optimally at 25–30 °C, at pH 7.0–7.5 and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SSK2-3 clustered with type strains of species of the genus , with which it exhibited 97.2–97.8 % 16S rRNA gene sequence similarity. Sequence similarity between the isolate and the other strains used in the phylogenetic analysis was <95.6 %. Strain SSK2-3 contained MK-6 as the predominant menaquinone and iso-C, iso-C G and C as the major fatty acids. The major polar lipids of strain SSK2-3 were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-3 was 32.4 mol%. DNA–DNA relatedness between the isolate and KCTC 12570, DSM 18792 and HWR-17 was 19, 15 and 20 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SSK2-3 is separate from other members of the genus . On the basis of the data presented, strain SSK2-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SSK2-3 ( = KCTC 23958  = CCUG 62414). An emended description of the genus is given.

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources (Award 11-2008-00-002-00)
  • BK21 Program from the Ministry of Education, Science and Technology (MEST) of South Korea
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2013-04-01
2021-08-01
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