1887

Abstract

A Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain xj7, was isolated from roots of L. growing in Beijing Cuihu Wetland, China. The isolate was identified as a member of the genus based on phenotypic characteristics and phylogenetic inference. The novel strain was spore-forming, motile, catalase-positive and oxidase-negative. Optimal growth of strain xj7 occurred at 28–30 °C and pH 7.0–7.5. Diphosphatidylglycerol was the most abundant polar lipid and occurred along with phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and three unknown aminophospholipids. The diamino acid found in the cell-wall peptidoglycan was -diaminopimelic acid. The predominant isoprenoid quinone was MK-7. The major fatty acid components were anteiso-C (56.1 %), iso-C (9.1 %), C (8.0 %), iso-C (6.3 %) and iso-C (5.1 %). The G+C content of genomic DNA was 47.9 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain xj7 fell within the evolutionary radiation encompassed by the genus , its closest neighbours were KK19 (97.5 %) and DSM 1735 (97.1 %). However, the DNA–DNA relatedness values between strain xj7 and KK19 and between strain xj7 and DSM 1735, were both 35 %. Based on phenotypic, chemotaxonomic and phylogenetic properties, strain xj7 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is xj7 ( = CGMCC 1.11012 = DSM 25190).

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2013-03-01
2019-10-23
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