1887

Abstract

A novel Gram-positive, slightly halophilic bacterium, designated strain B48, was isolated from soil around the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain B48 were non-motile rods and produced ellipsoidal endospores at a central or subterminal position in swollen sporangia. Strain B48 was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–10.0 % (w/v), with optimum growth occurring at 2.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain B48 was shown to belong to the genus within the phylum and showed the closest phylogenetic similarity to the species CV53 (97.4 %) and DS22 (96.9 %). The DNA G+C content of this new isolate was 40.1 mol%. The major cellular fatty acids of strain B48 were iso-C and anteiso-C, and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an aminophospholipid and two unknown phospholipids. The only quinone present was menaquinone 7 (MK-7). The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. All these features confirm the placement of isolate B48 within the genus . DNA–DNA hybridization experiments revealed a low level of relatedness between strain B48 and IBRC-M 10625 (8.1 %). On the basis of polyphasic evidence from this study, a new species of the genus , sp. nov., is proposed, with strain B48 ( = IBRC-M 10115 = DSM 25386 = CECT 8001) as the type strain.

Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • International Foundation for Science
  • IFS (Award A/4527-1)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • European Funds (FEDER)
  • National Science Foundation (Award grant DEB-0919290)
  • Junta de Andalucía (Award P10-CVI-6226)
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2013-04-01
2024-04-24
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References

  1. Arahal D. R., Ventosa A. ( 2002 ). Moderately halophilic and halotolerant species of Bacillus and related genera. . In Applications and Systematics of Bacillus and Relatives, pp. 8399. Edited by Berkeley R. C. W., Heyndrickx M., Logan N. A., de Vos P. . Oxford:: Blackwell;. [View Article]
    [Google Scholar]
  2. Atlas R. M. ( 2005 ). Handbook of Media for Environmental Microbiology, , 2nd edn.. Boca Raton, FL:: CRC Press;. [View Article]
    [Google Scholar]
  3. Baron E. J., Finegold S. M. ( 1990 ). Bailey and Scott's Diagnostic Microbiology, , 8 edn. . St. Louis:: Mosby;.
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  5. de la Haba R. R., Sánchez-Porro C., Marquez C., Ventosa A. ( 2011 ). Taxonomy of halophiles. . In Extremophiles Handbook. Edited by Horikoshi K. . Tokyo:: Springer;. [View Article]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  8. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  9. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [View Article] [PubMed]
    [Google Scholar]
  10. Harrigan W. F., McCance M. E. ( 1976 ). Laboratory Methods in Food and Dairy Microbiology. London:: Academic Press;.
    [Google Scholar]
  11. Huss V., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article]
    [Google Scholar]
  12. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H. J., Tindall B. J. ( 2006 ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56, 781786. [View Article] [PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  14. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. et al. ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  15. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. ( 2004 ). ARB: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  16. Makhdoumi-Kakhki A., Amoozegar M. A., Kazemi B., Pašić L., Ventosa A. ( 2012 ). Prokaryotic diversity in Aran-Bidgol salt lake, the largest hypersaline playa in Iran. . Microbes Environ 27, 8793. [View Article] [PubMed]
    [Google Scholar]
  17. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  18. Márquez M. C., Sánchez-Porro C., Ventosa A. ( 2011 ). Halophilic and haloalkaliphilic, aerobic endospore-forming bacteria in soil. . In Endospore-forming Soil Bacteria, pp. 309339. Edited by Logan N. A., De Vos P. . Berlin:: Springer;. [View Article]
    [Google Scholar]
  19. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. ( 2002 ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25, 360375. [View Article] [PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+ C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  21. Monciardini P., Cavaletti L., Schumann P., Rohde M., Donadio S. ( 2003 ). Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria . . Int J Syst Evol Microbiol 53, 569576. [View Article] [PubMed]
    [Google Scholar]
  22. Murray R. G. E., Doetsch R. N., Robinow C. F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1984 ). Deleya halophila, a new species of moderately halophilic bacteria. . Int J Syst Bacteriol 34, 287292. [View Article]
    [Google Scholar]
  24. Rhuland L. E., Work E., Denman R., Hoare D. ( 1955 ). The behavior of the isomers of diaminopimelic acid on paper chromatograms. . J Am Chem Soc 77, 48444846. [View Article]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  27. Tiago I., Pires C., Mendes V., Morais P. V., da Costa M. S., Veríssimo A. ( 2006 ). Bacillus foraminis sp. nov., isolated from a non-saline alkaline groundwater. . Int J Syst Evol Microbiol 56, 25712574. [View Article] [PubMed]
    [Google Scholar]
  28. Vaz-Moreira I., Figueira V., Lopes A. R., Lobo-da-Cunha A., Spröer C., Schumann P., Nunes O. C., Manaia C. M. ( 2012 ). Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant. . Int J Syst Evol Microbiol 62, 7177. [View Article] [PubMed]
    [Google Scholar]
  29. Ventosa A. ( 2006 ). Unusual micro-organisms from unusual habitats: hypersaline environments. . In Prokaryotic Diversity: Mechanisms and significance, pp. 223254. Edited by Logan N. A., Lappin-Scott H. M., Oyston P. C. F. . Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  30. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982 ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128, 19591968.
    [Google Scholar]
  31. Ventosa A., Nieto J. J., Oren A. ( 1998 ). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62, 504544.[PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
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