1887

Abstract

Two novel Gram-stain-negative, chemoheterotrophic and strictly aerobic bacteria, strains GY2 and SPO729, were isolated from a tidal flat at Gwangyang Bay in Korea and a marine sponge sample from the Pacific Ocean, respectively. The two strains were halotolerant, catalase- and oxidase-positive, and non-motile rods. Optimum temperature and pH for growth of both strains were observed to be 35 °C and pH 7.0–7.5, but optimum salinity for strain SPO729 [2–3 % (w/v)] was slightly higher than that for strain GY2 (1–2 %). The major cellular fatty acids of both strains were C, iso-C, iso-C, iso-Cω9, Cω7, iso-C and iso-C 3-OH. The genomic DNA G+C contents of strains GY2 and SPO729 were 55.1 and 57.9 mol%, respectively, and ubiquinone 8 (Q-8) was detected as the sole respiratory quinone from the two strains. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains GY2 and SPO729 formed tight phyletic lineages with members of the genus . Strain GY2 was closely related to ABABA23 (98.2 %), strain SPO729 (98.0 %) and CN85 (97.0 %); strain SPO729 was closely related to ABABA23 (98.3 %) and CN85 (98.2 %). The DNA–DNA relatedness values of strain GY2 with ABABA23, strain SPO729 and CN85 were 40.0±2.1 %, 13.1±3.9 % and 16.2±5.8 %, respectively, whereas those of strain SPO729 with ABABA23 and CN85 were 48.0±4.0 % and 34.6±9.3 %, respectively. On the basis of phenotypic and molecular features, it is concluded that the two strains GY2 and SPO729 represent two novel species of the genus , for which the names sp. nov. and are proposed; the type strains are GY2 ( = KACC 16189 = JCM 17800) and SPO729 ( = KCCM 42667 = JCM 14507), respectively.

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2013-04-01
2019-10-23
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