1887

Abstract

Two novel Gram-stain-negative, chemoheterotrophic and strictly aerobic bacteria, strains GY2 and SPO729, were isolated from a tidal flat at Gwangyang Bay in Korea and a marine sponge sample from the Pacific Ocean, respectively. The two strains were halotolerant, catalase- and oxidase-positive, and non-motile rods. Optimum temperature and pH for growth of both strains were observed to be 35 °C and pH 7.0–7.5, but optimum salinity for strain SPO729 [2–3 % (w/v)] was slightly higher than that for strain GY2 (1–2 %). The major cellular fatty acids of both strains were C, iso-C, iso-C, iso-Cω9, Cω7, iso-C and iso-C 3-OH. The genomic DNA G+C contents of strains GY2 and SPO729 were 55.1 and 57.9 mol%, respectively, and ubiquinone 8 (Q-8) was detected as the sole respiratory quinone from the two strains. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains GY2 and SPO729 formed tight phyletic lineages with members of the genus . Strain GY2 was closely related to ABABA23 (98.2 %), strain SPO729 (98.0 %) and CN85 (97.0 %); strain SPO729 was closely related to ABABA23 (98.3 %) and CN85 (98.2 %). The DNA–DNA relatedness values of strain GY2 with ABABA23, strain SPO729 and CN85 were 40.0±2.1 %, 13.1±3.9 % and 16.2±5.8 %, respectively, whereas those of strain SPO729 with ABABA23 and CN85 were 48.0±4.0 % and 34.6±9.3 %, respectively. On the basis of phenotypic and molecular features, it is concluded that the two strains GY2 and SPO729 represent two novel species of the genus , for which the names sp. nov. and are proposed; the type strains are GY2 ( = KACC 16189 = JCM 17800) and SPO729 ( = KCCM 42667 = JCM 14507), respectively.

Funding
This study was supported by the:
  • Technology Development Program for Agriculture and Forestry (TDPAF)
  • Ministry for Agriculture, Forestry and Fisheries
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment
  • Marine and Extreme Genome Research Center Program
  • Ministry of Land, Transport and Maritime Affairs
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.042606-0
2013-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/4/1335.html?itemId=/content/journal/ijsem/10.1099/ijs.0.042606-0&mimeType=html&fmt=ahah

References

  1. Acar J. F. ( 1980 ). The disc susceptibility test. . In Antibiotics in Laboratory Medicine, pp. 2454. Edited by Lorian V. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [View Article] [PubMed]
    [Google Scholar]
  3. Baba A., Miyazaki M., Nagahama T., Nogi Y. ( 2011 ). Microbulbifer chitinilyticus sp. nov. and Microbulbifer okinawensis sp. nov., chitin-degrading bacteria isolated from mangrove forests. . Int J Syst Evol Microbiol 61, 22152220. [View Article] [PubMed]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  5. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  6. DeSantis T. Z. Jr, Hugenholtz P., Keller K., Brodie E. L., Larsen N., Piceno Y. M., Phan R., Andersen G. L. ( 2006 ). NAST: a multiple sequence alignment server for comparative analysis of 16S rRNA genes. . Nucleic Acids Res 34 (Web Server issue), W394-9. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein, J. (2002). phylip (phylogeny inference package), version 3.6a, Seattle: Department of Genetics, University of Washington, Seattle, WA, USA.
  8. Gomori G. ( 1955 ). Preparation of buffers for use in enzyme studies. . In Methods in Enzymology, vol. 1, pp. 138146. Edited by Colowick S. P., Kaplan N. O. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  10. González J. M., Mayer F., Moran M. A., Hodson R. E., Whitman W. B. ( 1997 ). Microbulbifer hydrolyticus gen. nov., sp. nov., and Marinobacterium georgiense gen. nov., sp. nov., two marine bacteria from a lignin-rich pulp mill waste enrichment community. . Int J Syst Bacteriol 47, 369376. [View Article] [PubMed]
    [Google Scholar]
  11. Jeffries C. D., Holtman D. F., Guse D. G. ( 1957 ). Rapid method for determining the activity of microorganisms on nucleic acids. . J Bacteriol 73, 590591.[PubMed]
    [Google Scholar]
  12. Jin H. M., Jeong H., Moon E.-J., Math R. K., Lee K., Kim H.-J., Jeon C. O., Oh T. K., Kim J. F. ( 2011a ). Complete genome sequence of the polycyclic aromatic hydrocarbon-degrading bacterium Alteromonas sp. strain SN2. . J Bacteriol 193, 42924293. [View Article] [PubMed]
    [Google Scholar]
  13. Jin H. M., Lee H. J., Kim J. M., Park M. S., Lee K., Jeon C. O. ( 2011b ). Litorimicrobium taeanense gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 13921396. [View Article] [PubMed]
    [Google Scholar]
  14. Jung J. Y., Kim J. M., Jin H. M., Kim S. Y., Park W., Jeon C. O. ( 2011 ). Litorimonas taeanensis gen. nov., sp. nov., isolated from a sandy beach. . Int J Syst Evol Microbiol 61, 15341538. [View Article] [PubMed]
    [Google Scholar]
  15. Kämpfer P., Arun A. B., Young C.-C., Rekha P. D., Martin K., Busse H.-J., Chen W.-M. ( 2012 ). Microbulbifer taiwanensis sp. nov., isolated from Lutao Island (Green Island) soil. . Int J Syst Evol Microbiol 62, 24852489. [View Article]
    [Google Scholar]
  16. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  17. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  18. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  19. Lee S. H., Shim J. K., Kim J. M., Choi H. K., Jeon C. O. ( 2011 ). Henriciella litoralis sp. nov., isolated from a tidal flat, transfer of Maribaculum marinum Lai et al. 2009 to the genus Henriciella as Henriciella aquimarina nom. nov. and emended description of the genus Henriciella . . Int J Syst Evol Microbiol 61, 722727. [View Article] [PubMed]
    [Google Scholar]
  20. Leifson E. ( 1963 ). Determination of carbohydrate metabolism of marine bacteria. . J Bacteriol 85, 11831184.[PubMed]
    [Google Scholar]
  21. Lu S., Park M., Ro H.-S., Lee D. S., Park W., Jeon C. O. ( 2006 ). Analysis of microbial communities using culture-dependent and culture-independent approaches in an anaerobic/aerobic SBR reactor. . J Microbiol 44, 155161.[PubMed]
    [Google Scholar]
  22. Miyazaki M., Nogi Y., Ohta Y., Hatada Y., Fujiwara Y., Ito S., Horikoshi K. ( 2008 ). Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment. . Int J Syst Evol Microbiol 58, 11281133. [View Article] [PubMed]
    [Google Scholar]
  23. Nishijima M., Takadera T., Imamura N., Kasai H., An K. D., Adachi K., Nagao T., Sano H., Yamasato K. ( 2009 ). Microbulbifer variabilis sp. nov. and Microbulbifer epialgicus sp. nov., isolated from Pacific marine algae, possess a rod–coccus cell cycle in association with the growth phase. . Int J Syst Evol Microbiol 59, 16961707. [View Article] [PubMed]
    [Google Scholar]
  24. Park Y. J., Park M. S., Lee S. H., Park W., Lee K., Jeon C. O. ( 2011 ). Luteimonas lutimaris sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 61, 27292733. [View Article] [PubMed]
    [Google Scholar]
  25. Rosselló-Mora R., Amann R. ( 2001 ). The species concept for prokaryotes. . FEMS Microbiol Rev 25, 3967. [View Article] [PubMed]
    [Google Scholar]
  26. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  27. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P. . Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  28. Stamatakis A., Ott M., Ludwig T. ( 2005 ). RAxML-OMP: An efficient program for phylogenetic inference on SMPs”. . In Proceedings of 8th International Conference on Parallel Computing Technologies (PaCT2005), Lecture Notes in Computer Science , 3506, 288302, Springer Verlag;.
    [Google Scholar]
  29. Tang S. K., Wang Y., Cai M., Lou K., Mao P. H., Jin X., Jiang C. L., Xu L. H., Li W. J. ( 2008 ). Microbulbifer halophilus sp. nov., a moderately halophilic bacterium from north-west China. . Int J Syst Evol Microbiol 58, 20362040. [View Article] [PubMed]
    [Google Scholar]
  30. Wang C. S., Wang Y., Xu X. W., Zhang D. S., Wu Y. H., Wu M. ( 2009 ). Microbulbifer donghaiensis sp. nov., isolated from marine sediment of the East China Sea. . Int J Syst Evol Microbiol 59, 545549. [View Article] [PubMed]
    [Google Scholar]
  31. Yoon J. H., Kim I. G., Shin D. Y., Kang K. H., Park Y. H. ( 2003a ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 5357. [View Article] [PubMed]
    [Google Scholar]
  32. Yoon J. H., Kim H., Kang K. H., Oh T. K., Park Y. H. ( 2003b ). Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov.. Int J Syst Evol Microbiol 53, 13571361. [View Article] [PubMed]
    [Google Scholar]
  33. Yoon J. H., Kim I. G., Oh T. K., Park Y. H. ( 2004 ). Microbulbifer maritimus sp. nov., isolated from an intertidal sediment from the Yellow Sea, Korea. . Int J Syst Evol Microbiol 54, 11111116. [View Article] [PubMed]
    [Google Scholar]
  34. Yoon J. H., Jung S. Y., Kang S. J., Oh T. K. ( 2007 ). Microbulbifer celer sp. nov., isolated from a marine solar saltern of the Yellow Sea in Korea. . Int J Syst Evol Microbiol 57, 23652369. [View Article] [PubMed]
    [Google Scholar]
  35. Yurkov V., Stackebrandt E., Holmes A., Fuerst J. A., Hugenholtz P., Golecki J., Gad’on N., Gorlenko V. M., Kompantseva E. I., Drews G. ( 1994 ). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44, 427434. [View Article] [PubMed]
    [Google Scholar]
  36. Zhang D. S., Huo Y. Y., Xu X. W., Wu Y. H., Wang C. S., Xu X. F., Wu M. ( 2012 ). Microbulbifer marinus sp. nov. and Microbulbifer yueqingensis sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 62, 505510. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.042606-0
Loading
/content/journal/ijsem/10.1099/ijs.0.042606-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error