1887

Abstract

A DNA–DNA hybridization experiment was carried out to clarify the relationship between and . The taxonomic standing of these two species was unclear so far because of the high 16S rRNA gene sequence similarity value (99.9 %). The DNA–DNA relatedness values between JCM 15906 and JCM 13913 were above 91 % (91–99 %). In addition, JCM 15906 exhibited high gene sequence similarity with JCM 13913 (100 %). The gene sequence analysis and the DNA–DNA relatedness values demonstrated that JCM 15906 and JCM 13913 are a single species. Based on these data, we propose as a later heterotypic synonym of .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.042531-0
2013-02-01
2019-12-09
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/454.html?itemId=/content/journal/ijsem/10.1099/ijs.0.042531-0&mimeType=html&fmt=ahah

References

  1. Collins M. D., Love D. N., Karjalainen J., Kanervo A., Forsblom B., Willems A., Stubbs S., Sarkiala E., Bailey G. D.. & other authors ( 1994;). Phylogenetic analysis of members of the genus Porphyromonas and description of Porphyromonas cangingivalis sp. nov. and Porphyromonas cansulci sp. nov.. Int J Syst Bacteriol 44:, 674–679. [CrossRef][PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  3. Hirasawa M., Takada K.. ( 1994;). Porphyromonas gingivicanis sp. nov. and Porphyromonas crevioricanis sp. nov., isolated from beagles. . Int J Syst Bacteriol 44:, 637–640. [CrossRef][PubMed]
    [Google Scholar]
  4. Kitahara M., Tsuchida S., Kawasumi K., Amao H., Sakamoto M., Benno Y., Ohkuma M.. ( 2011;). Bacteroides chinchillae sp. nov. and Bacteroides rodentium sp. nov., isolated from chinchilla (Chinchilla lanigera) faeces. . Int J Syst Evol Microbiol 61:, 877–881. [CrossRef][PubMed]
    [Google Scholar]
  5. Komagata K., Suzuki K.. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19:, 161–207. [CrossRef]
    [Google Scholar]
  6. Kuykendall L. D., Roy M. A., O’neill J. J., Devine T. E.. ( 1988;). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  7. Miller L. T.. ( 1982;). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  8. Sakamoto M., Ohkuma M.. ( 2010;). Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods. . J Med Microbiol 59:, 1293–1302. [CrossRef][PubMed]
    [Google Scholar]
  9. Sakamoto M., Ohkuma M.. ( 2011;). Identification and classification of the genus Bacteroides by multilocus sequence analysis. . Microbiology 157:, 3388–3397. [CrossRef][PubMed]
    [Google Scholar]
  10. Sakamoto M., Ohkuma M.. ( 2012;). Bacteroides sartorii is an earlier heterotypic synonym of Bacteroides chinchillae and has priority. . Int J Syst Evol Microbiol 62:, 1241–1244. [CrossRef][PubMed]
    [Google Scholar]
  11. Sakamoto M., Suzuki N., Benno Y.. ( 2010;). hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes. . Int J Syst Evol Microbiol 60:, 2984–2990. [CrossRef][PubMed]
    [Google Scholar]
  12. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.042531-0
Loading
/content/journal/ijsem/10.1099/ijs.0.042531-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error