Four previously unknown, Gram-negative, anaerobic coccal strains were isolated from the tongue biofilm of healthy human adults (ages 22–29 years). The isolates displayed all phenotypic characteristics of the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that the four strains were phylogenetically homogeneous and comprised a distinct novel lineage within the genus Veillonella. The production of major cellular fatty acids (C13 : 0 and C17 : 1ω8) was consistent with that of other members of the genus Veillonella. Based on these observations, strains B16T, A16, B4 and Y6 represent a novel species, for which the name Veillonella tobetsuensis sp. nov. is proposed, with the type strain B16T ( = JCM 17976T = ATCC BAA-2400T).
Published Online:
Funding
This study was supported by the:
Ministry of Education, Culture, Sports, Science and Technology of Japan
BladenH. A.,
MergenhagenS. E.(1964). Ultrastucture of Veillonella and morphological correlation of an outer membrane with particles associated with endotoxic activity. . J Bacteriol88, 1482–1492.[PubMed]
CarlierJ.-P.(2009). Genus I. Veillonella Prèvot 1933, 118AL emend. Mays, Holdeman, Moore, Rogosa and Johnson 1982, 35. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 1059–1065. Edited by
PaulD. V.,
GeorgeM. G.,
DorothyJ.,
NoelR. K.,
WolfgangL.,
FredA. R.,
Karl-HeinzS.,
WilliamB. W.
. Dordrecht, Heidelberg, London, New York:: Springer;.
CarlierJ.-P.,
MarchandinH.,
Jumas-BilakE.,
LorinV.,
HenryC.,
CarrièreC.,
Jean-PierreH.(2002).Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae
. . Int J Syst Evol Microbiol52, 983–986. [View Article][PubMed]
DelwicheE. A.,
PestkaJ. J.,
TortorelloM. L.(1985). The veillonellae: Gram-negative cocci with a unique physiology. . Annu Rev Microbiol39, 175–193. [View Article][PubMed]
HoltJ. G.,
KriegN. R.,
SneathP. H. A.,
StaleyJ. T.,
WilliamsS. T.(1994).Bergey’s Manual of Determinative Bacteriology, , 9th edn.. Baltimore:: Williams & Wilkins;.
IgarashiE.,
KamaguchiA.,
FujitaM.,
MiyakawaH.,
NakazawaF.(2009). Identification of oral species of the genus Veillonella by polymerase chain reaction. . Oral Microbiol Immunol24, 310–313. [View Article][PubMed]
KolenbranderP.(1992). The genus Veillonella
. . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 4, pp. 1022–1040. Edited by
DworkinM.,
FalkowS.,
RosenbergE.,
SchleiferK.-H.,
StackebrandtE.
. New York:: Springer;.
KraatzM.,
TarasD.(2008).Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti
. . Int J Syst Evol Microbiol58, 2755–2761. [View Article][PubMed]
MarchandinH.,
TeyssierC.,
Siméon De BuochbergM.,
Jean-PierreH.,
CarriereC.,
Jumas-BilakE.(2003). Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. . Microbiology149, 1493–1501. [View Article][PubMed]
MarchandinH.,
TeyssierC.,
Jumas-BilakE.,
RobertM.,
ArtiguesA. C.,
Jean-PierreH.(2005). Molecular identification of the first human isolate belonging to the Veillonella ratti–Veillonella criceti group based on 16S rDNA and dnaK gene sequencing. . Res Microbiol156, 603–607. [View Article][PubMed]
MashimaI.,
KamaguchiA.,
NakazawaF.(2011). The distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy young adults. . Curr Microbiol63, 403–407. [View Article][PubMed]
MaysT. D.,
HoldemanL. V.,
MooreW. E. C.,
RogosaM.,
JohnsonJ. L.(1982). Taxnomy of Genus Veillonella Prévot. . Int J Syst Bacteriol32, 28–36. [View Article]
OlsenG. J.,
MatsudaH.,
HagstromR.,
OverbeekR.(1994). fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. . Comput Appl Biosci10, 41–48.[PubMed]
RogosaM.(1984). Anaerobic Gram-negative cocci. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 680–685. Edited by
KriegN. R.,
HoltJ. G.
. Baltimore:: Williams & Wilkins;.
TangermanA.,
NagengastF. M.(1996). A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method. . Anal Biochem236, 1–8. [View Article][PubMed]