1887

Abstract

Four previously unknown, Gram-negative, anaerobic coccal strains were isolated from the tongue biofilm of healthy human adults (ages 22–29 years). The isolates displayed all phenotypic characteristics of the genus . Comparative analysis of the 16S rRNA, and gene sequences indicated that the four strains were phylogenetically homogeneous and comprised a distinct novel lineage within the genus . The production of major cellular fatty acids (C and Cω8) was consistent with that of other members of the genus . Based on these observations, strains B16, A16, B4 and Y6 represent a novel species, for which the name sp. nov. is proposed, with the type strain B16 ( = JCM 17976 = ATCC BAA-2400).

Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science and Technology of Japan
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2013-04-01
2024-11-03
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References

  1. Arif N., Do T., Byun R., Sheehy E., Clark D., Gilbert S. C., Beighton D. ( 2008 ). Veillonella rogosae sp. nov., an anaerobic, Gram-negative coccus isolated from dental plaque. . Int J Syst Evol Microbiol 58, 581584. [View Article] [PubMed]
    [Google Scholar]
  2. Beighton D., Clark D., Hanakuka B., Gilbert S., Do T. ( 2008 ). The predominant cultivable Veillonella spp. of the tongue of healthy adults identified using rpoB sequencing. . Oral Microbiol Immunol 23, 344347. [View Article] [PubMed]
    [Google Scholar]
  3. Bladen H. A., Mergenhagen S. E. ( 1964 ). Ultrastucture of Veillonella and morphological correlation of an outer membrane with particles associated with endotoxic activity. . J Bacteriol 88, 14821492.[PubMed]
    [Google Scholar]
  4. Byun R., Carlier J.-P., Jacques N. A., Marchandin H., Hunter N. ( 2007 ). Veillonella denticariosi sp. nov., isolated from human carious dentine. . Int J Syst Evol Microbiol 57, 28442848. [View Article] [PubMed]
    [Google Scholar]
  5. Carlier J.-P. ( 2009 ). Genus I. Veillonella Prèvot 1933, 118AL emend. Mays, Holdeman, Moore, Rogosa and Johnson 1982, 35. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 10591065. Edited by Paul D. V., George M. G., Dorothy J., Noel R. K., Wolfgang L., Fred A. R., Karl-Heinz S., William B. W. . Dordrecht, Heidelberg, London, New York:: Springer;.
    [Google Scholar]
  6. Carlier J.-P., Marchandin H., Jumas-Bilak E., Lorin V., Henry C., Carrière C., Jean-Pierre H. ( 2002 ). Anaeroglobus geminatus gen. nov., sp. nov., a novel member of the family Veillonellaceae . . Int J Syst Evol Microbiol 52, 983986. [View Article] [PubMed]
    [Google Scholar]
  7. Delwiche E. A., Pestka J. J., Tortorello M. L. ( 1985 ). The veillonellae: Gram-negative cocci with a unique physiology. . Annu Rev Microbiol 39, 175193. [View Article] [PubMed]
    [Google Scholar]
  8. Holt J. G., Krieg N. R., Sneath P. H. A., Staley J. T., Williams S. T. ( 1994 ). Bergey’s Manual of Determinative Bacteriology, , 9th edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  9. Igarashi E., Kamaguchi A., Fujita M., Miyakawa H., Nakazawa F. ( 2009 ). Identification of oral species of the genus Veillonella by polymerase chain reaction. . Oral Microbiol Immunol 24, 310313. [View Article] [PubMed]
    [Google Scholar]
  10. Jumas-Bilak E., Carlier J.-P., Jean-Pierre H., Teyssier C., Gay B., Campos J., Marchandin H. ( 2004 ). Veillonella montpellierensis sp. nov., a novel, anaerobic, Gram-negative coccus isolated from human clinical samples. . Int J Syst Evol Microbiol 54, 13111316. [View Article] [PubMed]
    [Google Scholar]
  11. Kolenbrander P. ( 1992 ). The genus Veillonella . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 4, pp. 10221040. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. . New York:: Springer;.
    [Google Scholar]
  12. Kraatz M., Taras D. ( 2008 ). Veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of Veillonella ratti . . Int J Syst Evol Microbiol 58, 27552761. [View Article] [PubMed]
    [Google Scholar]
  13. Marchandin H., Jean-Pierre H., Carrière C., Canovas F., Darbas H., Jumas-Bilak E. ( 2001 ). Prosthetic joint infection due to Veillonella dispar . . Eur J Clin Microbiol Infect Dis 20, 340342.[PubMed]
    [Google Scholar]
  14. Marchandin H., Teyssier C., Siméon De Buochberg M., Jean-Pierre H., Carriere C., Jumas-Bilak E. ( 2003 ). Intra-chromosomal heterogeneity between the four 16S rRNA gene copies in the genus Veillonella: implications for phylogeny and taxonomy. . Microbiology 149, 14931501. [View Article] [PubMed]
    [Google Scholar]
  15. Marchandin H., Teyssier C., Jumas-Bilak E., Robert M., Artigues A. C., Jean-Pierre H. ( 2005 ). Molecular identification of the first human isolate belonging to the Veillonella ratti–Veillonella criceti group based on 16S rDNA and dnaK gene sequencing. . Res Microbiol 156, 603607. [View Article] [PubMed]
    [Google Scholar]
  16. Mashima I., Kamaguchi A., Nakazawa F. ( 2011 ). The distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy young adults. . Curr Microbiol 63, 403407. [View Article] [PubMed]
    [Google Scholar]
  17. Mays T. D., Holdeman L. V., Moore W. E. C., Rogosa M., Johnson J. L. ( 1982 ). Taxnomy of Genus Veillonella Prévot. . Int J Syst Bacteriol 32, 2836. [View Article]
    [Google Scholar]
  18. Olsen G. J., Matsuda H., Hagstrom R., Overbeek R. ( 1994 ). fastDNAmL: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. . Comput Appl Biosci 10, 4148.[PubMed]
    [Google Scholar]
  19. Paster B. J., Boches S. K., Galvin J. L., Ericson R. E., Lau C. N., Levanos V. A., Sahasrabudhe A., Dewhirst F. E. ( 2001 ). Bacterial diversity in human subgingival plaque. . J Bacteriol 183, 37703783. [View Article] [PubMed]
    [Google Scholar]
  20. Rogosa M. ( 1956 ). A selective medium for the isolation and enumeration of the Veillonella from the oral cavity. . J Bacteriol 72, 533536.[PubMed]
    [Google Scholar]
  21. Rogosa M. ( 1965 ). The genus Veillonella IV. Serological groupings, and genus and species emendations. . J Bacteriol 90, 704709.[PubMed]
    [Google Scholar]
  22. Rogosa M. ( 1984 ). Anaerobic Gram-negative cocci. . In Bergey’s Manual of Systematic Bacteriology, vol. 1, pp. 680685. Edited by Krieg N. R., Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  23. Rovery C., Etienne A., Foucault C., Berger P., Brouqui P. ( 2005 ). Veillonella montpellierensis endocarditis. . Emerg Infect Dis 11, 11121114. [View Article] [PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  25. Sato T., Sato M., Matsuyama J., Hoshino E. ( 1997 ). PCR-restriction fragment length polymorphism analysis of genes coding for 16S rRNA in Veillonella spp. . Int J Syst Bacteriol 47, 12681270. [View Article] [PubMed]
    [Google Scholar]
  26. Sutter V. L. ( 1984 ). Anaerobes as normal oral flora. . Rev Infect Dis 6 (Suppl. 1), S62S66. [View Article] [PubMed]
    [Google Scholar]
  27. Tangerman A., Nagengast F. M. ( 1996 ). A gas chromatographic analysis of fecal short-chain fatty acids, using the direct injection method. . Anal Biochem 236, 18. [View Article] [PubMed]
    [Google Scholar]
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