A strictly aerobic, Gram-stain-positive actinobacterial strain was isolated from a rhizosphere soil of a wild plant (Peucedanum japonicum Thumb.) collected on Mara Island, Jeju, Republic of Korea. Cells of strain RS-50T were oxidase-negative, catalase-positive, short rods and motile by means of a polar flagellum; the colonies were orange, circular, smooth and convex. meso-Diaminopimelic acid and glucose were the diagnostic diamino acid in the cell wall and the whole-cell sugar, respectively. The major menaquinone was MK-9(H4). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown phospholipids and an unknown lipid. The major fatty acids were iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content was 73.6 mol%. In 16S rRNA gene sequence-based phylogenetic trees, strain RS-50T formed a tight cluster with Angustibacter luteus (99.2 % sequence similarity); both were loosely related to the suborders Kineosporiineae and Micrococcineae. The DNA–DNA relatedness value of the isolate to A. luteus KACC 14249T was 22.3±0.9 %. On the basis of the results of phenotypic analyses and DNA–DNA hybridization experiments, strain RS-50T is considered to represent a novel species of the genus Angustibacter, for which the name Angustibacter peucedani sp. nov. is proposed. The type strain is RS-50T ( = KCTC 19628T = DSM 45329T). The description of the genus Angustibacter is emended.
Published Online:
Funding
This study was supported by the:
21C Frontier Microbial Genomics and Application Center Program, Ministry of Science & Technology
BeckerB.,
LechevalierM. P.,
LechevalierH. A.(1965). Chemical composition of cell-wall preparation from strains of various form-genera of aerobic actinomycetes. . Appl Microbiol13, 236–243.[PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39, 224–229. [View Article]
GordonR. E.,
BarnettD. A.,
HanderhanJ. E.,
PangC. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol24, 54–63. [View Article]
HopwoodD. A.,
BibbM. J.,
ChaterK. F.,
KieserT.,
BrutonC. J.,
KieserH. M.,
LydiateD. J.,
SmithC. P.,
WardJ. M.,
SchrempfH.(1985).Genetic Manipulation of Streptomyces. a laboratory manual. Norwich:: The John Innes Foundation;.
JukesT. H.,
CantorC. R.(1969). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21–132. Edited by
MunroH. N.
. New York:: Academic Press;.
KroppenstedtR. M.(1985). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics, pp. 173–199. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
LeeS. D.,
KangS.-O.,
HahY. C.(2000).Hongia gen. nov., a new genus of the order Actinomycetales
. . Int J Syst Evol Microbiol50, 191–199. [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MinnikinD. E.,
PatelP. V.,
AlshamaonyL.,
GoodfellowM.(1977). Polar lipid composition in the classification of Nocardia and related bacteria. . Int J Syst Bacteriol27, 104–117. [View Article]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]