1887

Abstract

Two non-motile, rod-shaped gammaproteobacteria were isolated from marine sponges collected from the coast of Japan at Numazu. The isolates were oxidase- and catalase-positive facultative anaerobes that fermented carbohydrates. They required sodium ions for growth and were slightly halophilic, growing in the presence of 1.0–5.0 % (w/v) NaCl (optimum of 2.0 % NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were Cω7, Cω7 and C and the hydroxy acids were C 3-OH and C 3-OH. The DNA G+C content was 48.3–48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5–96.9 % 16S rRNA gene sequence similarity with MKT110 and CL-33, with which the isolates formed a monophyletic cluster with 100 % bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus , which are aerobic, produce little or no menaquinone under aerobic conditions and possess different amounts of C and Cω7. Although some phenotypic differences were identified, the isolates should be assigned to the genus on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name sp. nov. is proposed. The type strain is HC50 ( = NBRC 108893  = DSM 25634). An emended description of the genus is presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.042077-0
2013-02-01
2020-02-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/709.html?itemId=/content/journal/ijsem/10.1099/ijs.0.042077-0&mimeType=html&fmt=ahah

References

  1. Bowman J. P., McCammon S. A., Nichols D. S., Skerratt J. H., Rea S. M., Nichols P. D., McMeekin T. A.. ( 1997;). Shewanella gelidimarina sp. nov. and Shewanella frigidimarina sp. nov., novel Antarctic species with the ability to produce eicosapentaenoic acid (20:5 ω 3) and grow anaerobically by dissimilatory Fe(III) reduction. . Int J Syst Bacteriol 47:, 1040–1047. [CrossRef][PubMed]
    [Google Scholar]
  2. Bozal N., Montes M. J., Tudela E., Jiménez F., Guinea J.. ( 2002;). Shewanella frigidimarina and Shewanella livingstonensis sp. nov. isolated from Antarctic coastal areas. . Int J Syst Evol Microbiol 52:, 195–205.[PubMed]
    [Google Scholar]
  3. Brettar I., Christen R., Höfle M. G.. ( 2002;). Rheinheimera baltica gen. nov., sp. nov., a blue-coloured bacterium isolated from the central Baltic Sea. . Int J Syst Evol Microbiol 52:, 1851–1857. [CrossRef][PubMed]
    [Google Scholar]
  4. Choi E. J., Kwon H. C., Sohn Y. C., Yang H. O.. ( 2010;). Kistimonas asteriae gen. nov., sp. nov., a gammaproteobacterium isolated from Asterias amurensis. . Int J Syst Evol Microbiol 60:, 938–943. [CrossRef][PubMed]
    [Google Scholar]
  5. Deming J. W., Somers L. K., Straube W. L., Swartz D. G., MacDonell M. T.. ( 1988;). Isolation of an obligately barophilic bacterium and description of a new genus, Colwellia gen. nov.. Syst Appl Microbiol 10:, 152–160. [CrossRef]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  7. Kurahashi M., Yokota A.. ( 2007;). Endozoicomonas elysicola gen. nov., sp. nov., a γ-proteobacterium isolated from the sea slug Elysia ornata. . Syst Appl Microbiol 30:, 202–206. [CrossRef][PubMed]
    [Google Scholar]
  8. Li H.-J., Zhang X.-Y., Zhang Y.-J., Zhou M.-Y., Gao Z.-M., Chen X.-L., Dang H.-Y., Zhang Y.-Z.. ( 2011;). Rheinheimera nanhaiensis sp. nov., isolated from marine sediments, and emended description of the genus Rheinheimera Brettar et al. 2002 emend. Merchant et al. 2007. . Int J Syst Evol Microbiol 61:, 1016–1022. [CrossRef][PubMed]
    [Google Scholar]
  9. Lin Y.-T., Shieh W. Y.. ( 2006;). Zobellella denitrificans gen. nov., sp. nov. and Zobellella taiwanensis sp. nov., denitrifying bacteria capable of fermentative metabolism. . Int J Syst Evol Microbiol 56:, 1209–1215. [CrossRef][PubMed]
    [Google Scholar]
  10. Nishijima M., Araki-Sakai M., Sano H.. ( 1997;). Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. . J Microbiol Methods 28:, 113–122. [CrossRef]
    [Google Scholar]
  11. Nishijima M., Takadera T., Imamura N., Kasai H., An K. D., Adachi K., Nagao T., Sano H., Yamasato K.. ( 2009;). Microbulbifer variabilis sp. nov. and Microbulbifer epialgicus sp. nov., isolated from Pacific marine algae, possess a rod–coccus cell cycle in association with the growth phase. . Int J Syst Evol Microbiol 59:, 1696–1707. [CrossRef][PubMed]
    [Google Scholar]
  12. Yang C.-S., Chen M.-H., Arun A. B., Chen C. A., Wang J.-T., Chen W.-M.. ( 2010;). Endozoicomonas montiporae sp. nov., isolated from the encrusting pore coral Montipora aequituberculata. . Int J Syst Evol Microbiol 60:, 1158–1162. [CrossRef][PubMed]
    [Google Scholar]
  13. Yi H., Song J., Cho J.-C., Chun J.. ( 2011;). Zobellella aerophila sp. nov., isolated from seashore sand, and emended description of the genus Zobellella. . Int J Syst Evol Microbiol 61:, 2491–2495. [CrossRef][PubMed]
    [Google Scholar]
  14. Zhang D.-C., Yu Y., Xin Y.-H., Liu H.-C., Zhou P.-J., Zhou Y.-G.. ( 2008;). Colwellia polaris sp. nov., a psychrotolerant bacterium isolated from Arctic sea ice. . Int J Syst Evol Microbiol 58:, 1931–1934. [CrossRef][PubMed]
    [Google Scholar]
  15. Zhao J.-S., Manno D., Leggiadro C., O’Neil D., Hawari J.. ( 2006;). Shewanella halifaxensis sp. nov., a novel obligately respiratory and denitrifying psychrophile. . Int J Syst Evol Microbiol 56:, 205–212. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.042077-0
Loading
/content/journal/ijsem/10.1099/ijs.0.042077-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error