1887

Abstract

Two non-motile, rod-shaped gammaproteobacteria were isolated from marine sponges collected from the coast of Japan at Numazu. The isolates were oxidase- and catalase-positive facultative anaerobes that fermented carbohydrates. They required sodium ions for growth and were slightly halophilic, growing in the presence of 1.0–5.0 % (w/v) NaCl (optimum of 2.0 % NaCl). Under aerobic conditions, the major isoprenoid quinones were ubiquinone-9 and menaquinone-9 and the minor quinones were ubiquinone-8 and menaquinone-8. The major cellular fatty acids were Cω7, Cω7 and C and the hydroxy acids were C 3-OH and C 3-OH. The DNA G+C content was 48.3–48.7 mol%. Phylogenetic analysis of 16S rRNA gene sequences placed the isolates within the radiation of the genus in a broad clade of uncultured clones recovered from various marine invertebrates. The isolates exhibited 96.5–96.9 % 16S rRNA gene sequence similarity with MKT110 and CL-33, with which the isolates formed a monophyletic cluster with 100 % bootstrap support. The phenotypic features (carbohydrate fermentation, quinone system and some major cellular fatty acids) differed from those of members of the genus , which are aerobic, produce little or no menaquinone under aerobic conditions and possess different amounts of C and Cω7. Although some phenotypic differences were identified, the isolates should be assigned to the genus on the basis of congruity of phylogeny and should be classified as representatives of a novel species, for which the name sp. nov. is proposed. The type strain is HC50 ( = NBRC 108893  = DSM 25634). An emended description of the genus is presented.

Funding
This study was supported by the:
  • , New Energy and Industrial Technology Development Organization of Japan (NEDO)
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2013-02-01
2021-03-08
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