Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_4

sp. nov., isolated from a batch reactor for the treatment of triphenylmethane dye effluent Free

Abstract

A Gram-staining-negative, non-endospore-forming, non-flagellated, non-motile and rod-shaped bacterium, designated strain LM2-5, was isolated from activated sludge in a sequencing batch reactor used for the treatment of triphenylmethane dye effluent. The taxonomy of strain LM2-5 was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain LM2-5 was aerobic, heterotrophic and positive for oxidase but negative for catalase activity. It grew at 16–37 °C (optimum 25 °C) and at pH 5.0–8.5 (optimum between pH 6.5 and pH 7.0). NaCl was not obligatory for growth but was tolerated at concentrations up to 2.0 % (w/v) NaCl. The novel strain formed yellow colonies on trypticase soy agar. Cells of strain LM2-5 were rods that measured 0.3–0.5 µm in width and 3.0–5.0 µm in length. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C and iso-Cω9. The genomic DNA G+C content was 66.7 mol%. In phylogenetic analyses based on 16S rRNA gene sequences, strain LM2-5 clustered with members of the genus and appeared most closely related to DS-123 (96.4 % sequence similarity), A3 (96.1 %), KIS28-6 (95.7 %) and Gsoil 191 (95.7 %). On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain LM2-5 was considered to represent a novel species of the genus , for which the name is proposed. The type strain is LM2-5 ( = CGMCC 1.7659  = JCM 15763).

  • Published Online:
Funding
This study was supported by the:
  • High-Tech Development Program 863 (Award 2006AA06Z316)
  • Chinese Academy of Sciences (Award KSCS2-YW-G-055-01)
© 2013 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.042002-0
2013-04-01
2025-01-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/4/1557.html?itemId=/content/journal/ijsem/10.1099/ijs.0.042002-0&mimeType=html&fmt=ahah

References

  1. Alderman D. J. ( 1985 ). Malachite green: a review. . J Fish Dis 8, 289–298. [View Article]
    [Google Scholar]
  2. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  3. Cowan S. T., Steel K. J. ( 1965 ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
     [Google Scholar]
  4. Cunha S., Tiago I., Luísa Pires A., da Costa M. S., Veríssimo A. ( 2006 ). Dokdonella fugitiva sp. nov., a Gammaproteobacterium isolated from potting soil. . Syst Appl Microbiol 29, 191–196. [View Article] [PubMed]
    [Google Scholar]
  5. Dong X.-Z., Cai M.-Y. ( 2001 ). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press;.
     [Google Scholar]
  6. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  8. Kim S. B., Falconer C., Williams E., Goodfellow M. ( 1998 ). Streptomyces thermocarboxydovorans sp. nov. and Streptomyces thermocarboxydus sp. nov., two moderately thermophilic carboxydotrophic species from soil. . Int J Syst Bacteriol 48, 59–68. [View Article] [PubMed]
    [Google Scholar]
  9. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  10. Kumar S., Tamura K., Nei M. ( 2004 ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5, 150–163. [View Article] [PubMed]
    [Google Scholar]
  11. Saddler G. S., Bradbury J. F. ( 2005 ). Order III. Xanthomonadales . . In Bergey’s Manual of Systematic Bacteriology (part B), , 2nd edn., vol. 2, pp. 63–122. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [View Article]
    [Google Scholar]
  12. Sly L. I., Blackall L. L., Kraat P. C., Tian-Shen T., Sangkhobol V. ( 1986 ). The use of second derivative plots for the determination of mol % guanine plus cytosine of DNA by the thermal denaturation method. . J Microbiol Methods 5, 139–156. [View Article]
    [Google Scholar]
  13. Srivastava S., Sinha R., Roy D. ( 2004 ). Toxicological effects of malachite green. . Aquat Toxicol 66, 319–329. [View Article] [PubMed]
    [Google Scholar]
  14. Ten L. N., Jung H. M., Im W. T., Oh H. W., Yang D. C., Yoo S. A., Lee S. T. ( 2009 ). Dokdonella ginsengisoli sp. nov., isolated from soil from a ginseng field, and emended description of the genus Dokdonella . . Int J Syst Evol Microbiol 59, 1947–1952. [View Article] [PubMed]
    [Google Scholar]
  15. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 4673–4680. [View Article] [PubMed]
    [Google Scholar]
  16. Yoo S. H., Weon H. Y., Anandham R., Kim B. Y., Hong S. B., Jeon Y. A., Koo B. S., Kwon S. W. ( 2009 ). Dokdonella soli sp. nov., a gammaproteobacterium isolated from soil. . Int J Syst Evol Microbiol 59, 1965–1968. [View Article] [PubMed]
    [Google Scholar]
  17. Yoon J. H., Kang S. J., Oh T. K. ( 2006 ). Dokdonella koreensis gen. nov., sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56, 145–150. [View Article] [PubMed]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.042002-0
Loading
Dokdonella immobilis sp. nov., isolated from a batch reactor for the treatment of triphenylmethane dye effluent
Int J Syst Evol Microbiol 63, 1557 (2013); https://doi.org/10.1099/ijs.0.042002-0
/content/journal/ijsem/10.1099/ijs.0.042002-0
/content/journal/ijsem/10.1099/ijs.0.042002-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error