A Gram-negative, motile, aerobic, oxidase- and catalase-positive rod, designated P10-2-4T, was isolated from an intertidal sand sample collected from a coastal area of Qingdao (Yellow Sea), China. The isolate reduced nitrate to nitrite and grew at 4–33 °C and with 0.5–12 % (w/v) NaCl. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C18 : 1ω7c and C16 : 0. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The major respiratory quinone was Q-8. The genomic DNA G+C content was 45.1 %. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain P10-2-4T belonged to the genus Neptunomonas. The isolate shared the highest 16S rRNA gene sequence similarity (98.1 %) with Neptunomonas japonica JAMM 0745T and 96.9, 96.5 and 95.9 % sequence similarities with N. antarctica S3-22T, N. concharum LHW37T and N. naphthovorans NAG-2N-126T, respectively, strains of the other three recognized species in the genus. DNA–DNA relatedness between strain P10-2-4T and N. japonica JCM 14595T was 35.6 %. Furthermore, strain P10-2-4T could be distinguished from the representatives of the genus Neptunomonas by a combination of phenotypic characteristics, such as temperature and NaCl concentration for growth, nitrate reduction, DNase activity and assimilation of substrates. The data from this study suggests that strain P10-2-4T represents a novel species in the genus Neptunomonas, for which the name Neptunomonas qingdaonensis sp. nov. is proposed. The type strain is P10-2-4T ( = CGMCC 1.10971T = KCTC 23686T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 41176130, 41106161, 31170055, 31070061 and 31025001)
Hi-Tech Research and Development program of China
(Award 2012AA092103 and 2011AA090703)
Special Fund of China for Marine-scientific Research in the Public Interest
(Award 201005032-6)
Natural Science Foundation of Shandong Province, China
(Award ZR2009DZ002 and JQ200910)
Foundation for Young Excellent Scientists in Shandong Province, China
(Award BS2010SW015)
China Ocean Mineral Resources R & D Association (COMRA) Special Foundation
(Award DY125-15-T-05)
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