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Abstract

A strictly anaerobic, moderately thermophilic, halotolerant rod, designated BELH25, was isolated from a water sample of a Tunisian hot spring. Cells were non-motile, 2–6 µm long and 0.4–0.6 µm wide, appearing singly or in pairs. The isolate grew at 45–70 °C (optimum 55 °C), at pH 6.2–8.0 (optimum pH 7.0) and with 0–4 % NaCl (optimum 0–2.0 %). Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Strain BELH25 used cellobiose, fructose, galactose, glucose, maltose, mannose, sucrose, starch and yeast extract as electron donors. The main fermentation products from glucose metabolism were formate, acetate, ethanol and CO. The predominant cellular fatty acids were iso-C, iso-C and anteiso-C. The DNA G+C content was 37.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain BELH25 was most closely related to JW/MS-VS5 and AeB (92.2 and 92.1 % sequence similarity, respectively), and the isolate was positioned approximately equidistantly between these genera. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, strain BELH25 is proposed to be a member of a novel species of a novel genus within the order family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is BELH25 ( = DSM 24455 = JCM 17559).

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2013-06-01
2020-01-29
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References

  1. Altschul S. F. , Gish W. , Miller W. , Myers E. W. , Lipman D. J. . ( 1990; ). Basic local alignment search tool. . J Mol Biol 215:, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  2. Andreesen J. R. , Zindel U. , Dürre P. . ( 1985; ). Clostridium cylindrosporum (ex Barker and Beck 1942) nom. rev.. Int J Syst Bacteriol 35:, 206–208. [CrossRef]
    [Google Scholar]
  3. Balch W. E. , Fox G. E. , Magrum L. J. , Woese C. R. , Wolfe R. S. . ( 1979; ). Methanogens: reevaluation of a unique biological group. . Microbiol Rev 43:, 260–296.[PubMed]
    [Google Scholar]
  4. Bouanane-Darenfed A. , Fardeau M.-L. , Grégoire P. , Joseph M. , Kebbouche-Gana S. , Benayad T. , Hacene H. , Cayol J.-L. , Ollivier B. . ( 2011; ). Caldicoprobacter algeriensis sp. nov. a new thermophilic anaerobic, xylanolytic bacterium isolated from an Algerian hot spring. . Curr Microbiol 62:, 826–832. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cashion P. , Holder-Franklin M. A. , McCully J. , Franklin M. . ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81:, 461–466. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cord-Ruwisch R. . ( 1985; ). A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate-reducing bacteria. . J Microbiol Methods 4:, 33–36. [CrossRef]
    [Google Scholar]
  7. Edgar R. C. . ( 2004; ). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32:, 1792–1797. [CrossRef] [PubMed]
    [Google Scholar]
  8. Fardeau M.-L. , Ollivier B. , Patel B. K. C. , Magot M. , Thomas P. , Rimbault A. , Rocchiccioli F. , Garcia J.-L. . ( 1997; ). Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. . Int J Syst Bacteriol 47:, 1013–1019. [CrossRef] [PubMed]
    [Google Scholar]
  9. Fardeau M.-L. , Magot M. , Patel B. K. C. , Thomas P. , Garcia J.-L. , Ollivier B. . ( 2000; ). Thermoanaerobacter subterraneus sp. nov., a novel thermophile isolated from oilfield water. . Int J Syst Evol Microbiol 50:, 2141–2149. [CrossRef] [PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–787. [CrossRef]
    [Google Scholar]
  11. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  12. Haouari O. , Fardeau M.-L. , Cayol J.-L. , Fauque G. , Casiot C. , Elbaz-Poulichet F. , Hamdi M. , Ollivier B. . ( 2008; ). Thermodesulfovibrio hydrogeniphilus sp. nov., a new thermophilic sulphate-reducing bacterium isolated from a Tunisian hot spring. . Syst Appl Microbiol 31:, 38–42. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hungate R. E. . ( 1969; ). A roll -tube method for cultivation of strict anaerobes. . In Methods in Microbiology, vol. 3B, pp. 117–132. Edited by Norris J. R. , Ribbons D. W. . . New York:: Academic Press;. [CrossRef]
    [Google Scholar]
  14. Jukes T. H. , Cantor C. R. . ( 1969; ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . . New York:: Academic Press;.
    [Google Scholar]
  15. Khelifi N. , Ben Romdhane E. , Hedi A. , Postec A. , Fardeau M.-L. , Hamdi M. , Tholozan J.-L. , Ollivier B. , Hirshler-Réa A. . ( 2010; ). Characterization of Microaerobacter geothermalis gen. nov., sp. nov., a novel microaerophilic, nitrate- and nitrite-reducing thermophilic bacterium isolated from a terrestrial hot spring in Tunisia. . Extremophiles 14:, 297–304. [CrossRef]
    [Google Scholar]
  16. Kuykendall L. D. , Roy M. A. , O’Neil J. J. , Devine T. E. . ( 1988; ). Fatty acids, antibiotic resistance, and desoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38:, 358–361. [CrossRef]
    [Google Scholar]
  17. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  18. Miller L. T. . ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16:, 584–586.[PubMed]
    [Google Scholar]
  19. Ogg C. D. , Patel B. K. C. . ( 2010; ). Fervidicella metallireducens gen. nov., sp. nov., a thermophilic, anaerobic bacterium from geothermal waters. . Int J Syst Evol Microbiol 60:, 1394–1400. [CrossRef] [PubMed]
    [Google Scholar]
  20. Saitou N. , Nei M. . ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425. [CrossRef] [PubMed]
    [Google Scholar]
  21. Seyfried M. , Lyon D. , Rainey F. A. , Wiegel J. . ( 2002; ). Caloramator viterbensis sp. nov., a novel thermophilic, glycerol-fermenting bacterium isolated from a hot spring in Italy. . Int J Syst Evol Microbiol 52:, 1177–1184. [CrossRef] [PubMed]
    [Google Scholar]
  22. Van de Peer Y. , De Wachter R. . ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. . Comput Appl Biosci 10:, 569–570.[PubMed]
    [Google Scholar]
  23. Wiegel J. . ( 2009; ). Family I. Clostridiaceae . . In The Firmicutes, , 2nd edn., vol. 3, pp. 736–738. Edited by De Vos P. , Garrity G. M. , Jones D. , Krieg N. R. , Ludwig W. , Rainey F. A. , Schleifer K. H. , Whitman B. . . Dordrecht:: Springer;.
    [Google Scholar]
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