RT Journal Article SR Electronic(1) A1 Lee, Geun-Hye A1 Rhee, Moon-Soo A1 Chang, Dong-Ho A1 Lee, Jonghwan A1 Kim, Seil A1 Yoon, Min Ho A1 Kim, Byoung-ChanYR 2013 T1 Oscillibacterruminantium sp. nov., isolated from the rumen of Korean native cattle JF International Journal of Systematic and Evolutionary Microbiology, VO 63 IS Pt_6 SP 1942 OP 1946 DO https://doi.org/10.1099/ijs.0.041749-0 PB Microbiology Society, SN 1466-5034, AB A strictly anaerobic, Gram-negative, non-spore-forming bacterium, designated GH1T, was isolated from the rumen of Korean native cattle (HanWoo). Cells were straight to slightly curved rods (2.0–4.5 µm long) and were motile by peritrichous flagella. The isolate grew at 30–45 °C (optimum 40 °C), at pH 5.5–6.5 (optimum pH 6.0) and with up to 3.5 % (w/v) NaCl. Strain GH1T produced acid from d-glucose, d-ribose and d-xylose, with butyric acid being the major end product. The genomic DNA G+C content was 54.6 mol%. Based on comparative 16S rRNA gene sequence analysis, strain GH1T was most closely related to Oscillibacter valericigenes Sjm18-20T (97.3 % 16S rRNA gene sequence similarity). DNA–DNA hybridization between strain GH1T and O. valericigenes DSM 18026T showed 24 % reassociation. The major fatty acids were iso-C13 : 0 (13.0 %), iso-C15 : 0 (17.6 %), anteiso-C15 : 0 (8.4 %) and C14 : 0 (4.1 %), and the cellular fatty acid methyl esters as dimethylacetals (DMAs) were C16 : 0 DMA (17.8 %), iso-C15 : 0 DMA (15.2 %) and C14 : 0 DMA (4.52 %). The cell-wall peptidoglycan of strain GH1T contained meso-diaminopimelic acid and the major cell-wall sugar was galactose. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA–DNA relatedness and distinct phenotypic characteristics, strain GH1T is classified in the genus Oscillibacter as a member of a novel species, for which the name Oscillibacter ruminantium sp. nov. is proposed. The type strain is GH1T ( = KCTC 15176T = NBRC 108824T = JCM 18333T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.041749-0