1887

Abstract

A strictly anaerobic, Gram-negative, non-spore-forming bacterium, designated GH1, was isolated from the rumen of Korean native cattle (HanWoo). Cells were straight to slightly curved rods (2.0–4.5 µm long) and were motile by peritrichous flagella. The isolate grew at 30–45 °C (optimum 40 °C), at pH 5.5–6.5 (optimum pH 6.0) and with up to 3.5 % (w/v) NaCl. Strain GH1 produced acid from -glucose, -ribose and -xylose, with butyric acid being the major end product. The genomic DNA G+C content was 54.6 mol%. Based on comparative 16S rRNA gene sequence analysis, strain GH1 was most closely related to Sjm18-20 (97.3 % 16S rRNA gene sequence similarity). DNA–DNA hybridization between strain GH1 and DSM 18026 showed 24 % reassociation. The major fatty acids were iso-C (13.0 %), iso-C (17.6 %), anteiso-C (8.4 %) and C (4.1 %), and the cellular fatty acid methyl esters as dimethylacetals (DMAs) were C DMA (17.8 %), iso-C DMA (15.2 %) and C DMA (4.52 %). The cell-wall peptidoglycan of strain GH1 contained -diaminopimelic acid and the major cell-wall sugar was galactose. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA–DNA relatedness and distinct phenotypic characteristics, strain GH1 is classified in the genus as a member of a novel species, for which the name sp. nov. is proposed. The type strain is GH1 ( = KCTC 15176 = NBRC 108824 = JCM 18333).

Funding
This study was supported by the:
  • National Research Foundation of Korea (NRF) (Award OGM0211212)
  • Korea government (MEST (Award NMM0101232)
  • KRIBB Research Initiative Program
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2013-06-01
2024-03-28
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References

  1. Benson D., Lipman D. J., Ostell J. ( 1993 ). GenBank. . Nucleic Acids Res 21, 29632965. [View Article] [PubMed]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  3. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. ( 1994 ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  4. Hu H. Y., Lim B. R., Goto N., Fujie K. ( 2001 ). Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. . J Microbiol Methods 47, 1724. [View Article] [PubMed]
    [Google Scholar]
  5. Hungate R. E. ( 1969 ). A roll tube method for cultivation of strict anaerobes. . Methods Microbiol 3B, 117132. [View Article]
    [Google Scholar]
  6. Hwang W., Kim H., Lee E., Lim J., Roh S., Shin T., Hwang K., Lee B. ( 2000 ). Purification and embryotropic roles of tissue inhibitor of metalloproteinase-1 in development of ‘HanWoo’ (Bos taurus coreanae) oocytes co-cultured with bovine oviduct epithelial cells. . J Vet Med Sci 62, 15. [View Article] [PubMed]
    [Google Scholar]
  7. Iino T., Mori K., Tanaka K., Suzuki K. I., Harayama S. ( 2007 ). Oscillibacter valericigenes gen. nov., sp. nov., a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam. . Int J Syst Evol Microbiol 57, 18401845. [View Article] [PubMed]
    [Google Scholar]
  8. Kamra D. N. ( 2005 ). Rumen microbial ecosystem. . Curr Sci 89, 124135.
    [Google Scholar]
  9. Lee J. Y., Hwang B. K. ( 2002 ). Diversity of antifungal actinomycetes in various vegetative soils of Korea. . Can J Microbiol 48, 407417. [View Article] [PubMed]
    [Google Scholar]
  10. Mackie R. I., Aminov R. I., Hu W., Klieve A. V., Ouwerkerk D., Sundset M. A., Kamagata Y. ( 2003 ). Ecology of uncultivated Oscillospira species in the rumen of cattle, sheep, and reindeer as assessed by microscopy and molecular approaches. . Appl Environ Microbiol 69, 68086815. [View Article] [PubMed]
    [Google Scholar]
  11. Madrid V. M., Taylor G. T., Scranton M. I., Chistoserdov A. Y. ( 2001 ). Phylogenetic diversity of bacterial and archaeal communities in the anoxic zone of the Cariaco Basin. . Appl Environ Microbiol 67, 16631674. [View Article] [PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  13. Miron J., Ben-Ghedalia D., Morrison M. ( 2001 ). Invited review: adhesion mechanisms of rumen cellulolytic bacteria. . J Dairy Sci 84, 12941309. [View Article] [PubMed]
    [Google Scholar]
  14. Pers-Kamczyc E., Zmora P., Cieślak A., Szumacher-Strabel M. ( 2011 ). Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research. . J Anim Feed Sci 20, 315337.
    [Google Scholar]
  15. Rainey F. A. ( 2009 ). Family VIII. Ruminococcaceae fam. nov. . In Bergey’s Manual of Systematic Bacteriology, 2nd edn, pp. 1016–1018. Edited by Vos P. D., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B. . New York:: Springer;.
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  18. Schön R., Groth I. ( 2006 ). Practical thin layer chromatography techniques for diaminopimelic acid and whole cell sugar analyses in the classification of environmental actinomycetes. . J Basic Microbiol 46, 243249. [View Article] [PubMed]
    [Google Scholar]
  19. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  20. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  22. Van Soest P. J. ( 1994 ). Nutritional ecology of the ruminant, , 2nd edn.. Ithaca, New York:: Cornell University Press;.
    [Google Scholar]
  23. Wallace W. H., Gates J. E. ( 1986 ). Identification of eubacteria isolated from leaf cavities of four species of the N-fixing azolla fern as arthrobacter conn and dimmick. . Appl Environ Microbiol 52, 425429.[PubMed]
    [Google Scholar]
  24. Widdel F., Kohring G. W., Mayer F. ( 1983 ). Studies on dissimilatory sulfate-reducing bacteria that decompose fatty acids. . Arch Microbiol 134, 286294. [View Article]
    [Google Scholar]
  25. Wolin E. A., Wolin M. J., Wolfe R. S. ( 1963 ). Formation of methane by bacterial extracts. . J Biol Chem 238, 28822886.[PubMed]
    [Google Scholar]
  26. Yanagita K., Manome A., Meng X.-Y., Hanada S., Kanagawa T., Tsuchida T., Mackie R. I., Kamagata Y. ( 2003 ). Flow cytometric sorting, phylogenetic analysis and in situ detection of Oscillospira guilliermondii, a large morphologically conspicuous but uncultured ruminal bacterium. . Int J Syst Evol Microbiol 53, 16091614. [View Article] [PubMed]
    [Google Scholar]
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