A polyphasic analysis was undertaken of seven independent isolates of Gram-negative cocci collected from pathological clinical samples from New York, Louisiana, Florida and Illinois and healthy subgingival plaque from a patient in Virginia, USA. The 16S rRNA gene sequence similarity among these isolates was 99.7–100 %, and the closest species with a validly published name was Neisseria lactamica (96.9 % similarity to the type strain). DNA–DNA hybridization confirmed that these isolates are of the same species and are distinct from their nearest phylogenetic neighbour, N. lactamica. Phylogenetic analysis of 16S and 23S rRNA gene sequences indicated that the novel species belongs in the genus Neisseria. The predominant cellular fatty acids were C16 : 0, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C18 : 1ω7c. The cellular fatty acid profile, together with other phenotypic characters, further supports the inclusion of the novel species in the genus Neisseria. The name Neisseria oralis sp. nov. (type strain 6332T = DSM 25276T = LMG 26725T) is proposed.
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res25, 3389–3402. [View Article][PubMed]
AnisimovaM.,
GascuelO.(2006). Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. . Syst Biol55, 539–552. [View Article][PubMed]
ChunJ.,
LeeJ. H.,
JungY.,
KimM.,
KimS.,
KimB. K.,
LimY. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol57, 2259–2261. [View Article][PubMed]
EzakiT.,
HashimotoY.,
YabuuchiE.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol39, 224–229. [View Article]
ForbesB. A.,
SahmD. F.,
WeissfeldA. S.(1998). Overview of bacterial identification methods and strategies. . In Bailey and Scott’s Diagnostic Microbiology, pp. 424–446. Edited by
RocheJ.
. St Louis, MO:: Mosby;.
GriceE. A.,
KongH. H.,
ConlanS.,
DemingC. B.,
DavisJ.,
YoungA. C.,
BouffardG. G.,
BlakesleyR. W.,
MurrayP. R. et al.(2009). Topographical and temporal diversity of the human skin microbiome. . Science324, 1190–1192. [View Article][PubMed]
GuindonS.,
GascuelO.(2003). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol52, 696–704. [View Article][PubMed]
JandaW. M.,
GaydosC. A.(2007).Neisseria
. . In Manual of Clinical Microbiology, , 9th edn., pp. 601–620. Edited by
MurrayP. R.,
BaronE. J.,
JorgensenJ. H.,
LandryM. L.,
PfallerM. A.
. Washington, DC:: American Society for Microbiology;.
KohlerschmidtD. J.,
MusserK. A.,
DumasN. B.(2009). Identification of aerobic Gram-negative bacteria. . In Practical Handbook of Microbiology, , 2nd edn., pp. 67–80. Edited by
GoldmanE.,
GreenL. H.
. Boca Raton, FL:: CRC Press;.
LoganN. A.,
LebbeL.,
HosteB.,
GorisJ.,
ForsythG.,
HeyndrickxM.,
MurrayB. L.,
SymeN.,
Wynn-WilliamsD. D.,
De VosP.(2000). Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillusfumarioli sp. nov.. Int J Syst Evol Microbiol50, 1741–1753.[PubMed]
NelsonK. E.,
WeinstockG. M.,
HighlanderS. K.,
WorleyK. C.,
CreasyH. H.,
WortmanJ. R.,
RuschD. B.,
MitrevaM.,
SodergrenE. et al.(2010). A catalog of reference genomes from the human microbiome. . Science328, 994–999. [View Article][PubMed]
RylevM.,
Bek-ThomsenM.,
ReinholdtJ.,
EnnibiO. K.,
KilianM.(2011). Microbiological and immunological characteristics of young Moroccan patients with aggressive periodontitis with and without detectable Aggregatibacter actinomycetemcomitans JP2 infection. . Mol Oral Microbiol26, 35–51. [View Article][PubMed]
StaudingerT.,
PipalA.,
RedlB.(2011). Molecular analysis of the prevalent microbiota of human male and female forehead skin compared to forearm skin and the influence of make-up. . J Appl Microbiol110, 1381–1389. [View Article][PubMed]
TønjumT.(2005). Family I. Neisseriaceae Prevot 1933, 119AL emend. Dewhirst, Paster and Bright 1989, 265. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2C, pp. 775–776. Edited by
GarrityG. M.,
BrennerD. J.,
KriegN. R.,
StaleyJ. T.
. New York:: Springer;.
van der GastC. J.,
WalkerA. W.,
StressmannF. A.,
RogersG. B.,
ScottP.,
DanielsT. W.,
CarrollM. P.,
ParkhillJ.,
BruceK. D.(2011). Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities. . ISME J5, 780–791. [View Article][PubMed]
VandammeP.,
HolmesB.,
BercovierH.,
CoenyeT.(2006). Classification of Centers for Disease Control Group Eugonic Fermenter (EF)-4a and EF-4b as Neisseria animaloris sp. nov. and Neisseria zoodegmatis sp. nov., respectively. . Int J Syst Evol Microbiol56, 1801–1805. [View Article][PubMed]
VelaA. I.,
CollinsM. D.,
LawsonP. A.,
GarcíaN.,
DomínguezL.,
Fernández-GarayzábalJ. F.(2005).Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs. . Int J Syst Evol Microbiol55, 643–647. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E. et al.(1987). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
WillemsA.,
Doignon-BourcierF.,
GorisJ.,
CoopmanR.,
de LajudieP.,
De VosP.,
GillisM.(2001). DNA–DNA hybridization study of Bradyrhizobium strains. . Int J Syst Evol Microbiol51, 1315–1322.[PubMed]