1887

Abstract

A novel Gram-positive, moderately halophilic bacterium, designated strain X4B, was isolated from soil around the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain X4B were motile rods and formed ellipsoidal endospores at a terminal or subterminal position in swollen sporangia. Strain X4B was a strictly aerobic bacterium, catalase- and oxidase-positive. The strain was able to grow at NaCl concentrations of 0.5–22.5 % (w/v), with optimum growth occurring at 7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35 °C and pH 7.0. Analysis of 16S rRNA gene sequence revealed that strain X4B is a member of the family , constituting a novel phyletic lineage within this family. Highest sequence similarities were obtained with the 16S rRNA gene sequences of the type strains of (96.0 %), (95.9 %), (95.8 %) and (95.7 %), respectively. The DNA G+C content of this novel isolate was 35.2 mol%. The major cellular fatty acids of strain X4B were anteiso-C and anteiso-C and its polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, two aminolipids, an aminophospholipid and an unknown phospholipid. The isoprenoid quinones were MK-7 (89 %) and MK-6 (11 %). The peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. On the basis of 16S rRNA gene sequence analysis in combination with chemotaxonomic and phenotypic data, strain X4B represents a novel species in a new genus in the family , order for which the name gen. nov., sp. nov. is proposed. The type strain of the type species () is X4B ( = IBRC-M 10629 = KCTC 13827).

Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • International Foundation for Science (IFS) (Award A/4527-1)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • Federación Española de Enfermedades Raras (FEDER)
  • National Science Foundation (Award DEB-0919290)
  • Junta de Andalucía (Award (P10-CVI-6226)
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2013-01-01
2021-10-26
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