1887

Abstract

A phytoplasma was identified in naturally infected wild plants exhibiting typical witches’ broom symptoms ( witches’ broom: BltWB) in Myanmar. The 16S rRNA gene sequence revealed that BltWB phytoplasma had the highest similarity to that of ‘ Phytoplasma ziziphi’ and it was also closely related to that of ‘ ’. Phylogenetic analysis of the 16S rRNA gene sequences indicated that the BltWB phytoplasma clustered as a discrete subclade with Elm yellows phytoplasmas. RFLP analysis of the 16S rRNA gene including the 16S–23S spacer region differentiated the BltWB phytoplasma from ‘. ’, ‘. ’ and ‘ ’. Analysis of additional ribosomal protein () and translocase protein () gene sequences and phylogenetic analysis of BltWB showed that this phytoplasma was clearly distinguished from those of other ‘ ’ taxa. Taking into consideration the unique plant host and the restricted geographical occurrence in addition to the 16S rRNA gene sequence similarity, the BltWB phytoplasma is proposed to represent a novel taxon, ‘ Phytoplasma balanitae’.

Funding
This study was supported by the:
  • , NRF grant
  • , MEST , (Award No. 2010-0027638)
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2013-02-01
2020-07-03
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