1887

Abstract

A phytoplasma was identified in naturally infected wild plants exhibiting typical witches’ broom symptoms ( witches’ broom: BltWB) in Myanmar. The 16S rRNA gene sequence revealed that BltWB phytoplasma had the highest similarity to that of ‘ Phytoplasma ziziphi’ and it was also closely related to that of ‘ Phytoplasma ulmi ’. Phylogenetic analysis of the 16S rRNA gene sequences indicated that the BltWB phytoplasma clustered as a discrete subclade with Elm yellows phytoplasmas. RFLP analysis of the 16S rRNA gene including the 16S–23S spacer region differentiated the BltWB phytoplasma from ‘. P. ziziphi ’, ‘. P. ulmi ’ and ‘ Phytoplasma trifolii ’. Analysis of additional ribosomal protein () and translocase protein () gene sequences and phylogenetic analysis of BltWB showed that this phytoplasma was clearly distinguished from those of other ‘ Phytoplasma ’ taxa. Taking into consideration the unique plant host and the restricted geographical occurrence in addition to the 16S rRNA gene sequence similarity, the BltWB phytoplasma is proposed to represent a novel taxon, ‘ Phytoplasma balanitae’.

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2013-02-01
2019-10-14
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