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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 63, Issue Pt_3

gen. nov., sp. nov., a novel member in the family isolated from deep-sea sediment in the Red Sea, Saudi Arabia Free

Abstract

A slow-growing, strictly aerobic, Gram-negative, coccus bacterial strain, designated KAUST100406-0324, was isolated from sea-floor sediment collected from the Red Sea, Saudi Arabia. The catalase- and oxidase-positive strain was non-sporulating and only slightly halophilic. Optimum growth occurred at 20–25 °C and at pH values ranging from 7.0 to 8.0. The major cellular fatty acids of the strain were unsaturated Cω6 and/or Cω7, Cω7 11-methyl and Cω7 and/or Cω6 The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. Ubiquinone 10 was the predominant lipoquinone. The DNA G+C content of strain KAUST100406-0324 was 64.0 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that the novel strain belonged to the family of the class but formed a distinct evolutionary lineage from other bacterial species with validly published names. The 16S rRNA gene sequence of the novel strain was distantly related, but formed a monophyletic cluster with, those of bacteria from two moderately halophilic genera, and . The similarity of the sequence between the novel strain KAUST100406-0324 and the type strains Y26 (accession number FJ230842), B5-6 (DQ514326) and CL-SP27 (AY906863) were 94.5 %, 95.2 % and 95.3 %, respectively. Based on the physiological, phylogenetic and chemotaxonomic characteristics presented in this study, we propose that this strain represents a novel species of a new genus in the family , for which the name of gen. nov., sp. nov. was proposed, with KAUST100406-0324 ( = JCM 17872  = NRRL B-59665) as the type strain.

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Funding
This study was supported by the:
  • KAUST (Award SA-C0040/UK-C001)
© 2013 IUMS
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2013-03-01
2025-01-25
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Profundibacterium mesophilum gen. nov., sp. nov., a novel member in the family Rhodobacteraceae isolated from deep-sea sediment in the Red Sea, Saudi Arabia
Int J Syst Evol Microbiol 63, 1007 (2013); https://doi.org/10.1099/ijs.0.041525-0
/content/journal/ijsem/10.1099/ijs.0.041525-0
/content/journal/ijsem/10.1099/ijs.0.041525-0
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