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Abstract

An endophytic actinomycete, designated strain I10A-01259, was isolated from a surface-sterilized fruit of (Rupr.) Maxim., a medicinal plant, which was collected from a suburb of Beijing, China. Whole-cell hydrolysates of the isolate contained galactose and -diaminopimelic acid. The predominant phospholipids were phosphatidylglycerol and diphosphatidylglycerol; the menaquinones consisted mainly of MK-9, MK-11 and MK-12, with a minor amount of MK-10. The major fatty acids were iso-C, anteiso-C and iso-C. Comparative analysis of 16S rRNA gene sequences indicated that strain I10A-01259 was most closely related to S186 (93.2 % sequence similarity), YIM 90462 (93.0 %) and other strains of genera within the families and . On the phylogenetic tree based on 16S rRNA gene sequences, strain I10A-01259 fell within the radius of the suborder , in which the strain formed a distinct lineage next to the genera of the families and . Based on the data from our polyphasic taxonomic study, a novel genus and species, gen. nov., sp. nov., are proposed within the suborder . The type strain of is strain I10A-01259 ( = CPCC 203428  = DSM 45601  = KCTC 19844).

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2013-03-01
2019-10-19
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References

  1. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  2. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  3. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  4. Goodfellow M., Stanton L. J., Simpson K. E., Minnikin D. E.. ( 1990;). Numerical and chemical classification of Actinoplanes and some related actinomycetes. . J Gen Microbiol 136:, 19–36. [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K.. ( 1997;). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47:, 1129–1133. [CrossRef][PubMed]
    [Google Scholar]
  6. Kim O. S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  7. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  8. Kimura M.. ( 1983;). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  9. Kluge A. G., Farris F. S.. ( 1969;). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  10. Kroppenstedt R. M.. ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by Goodfellow M., Minnikin D. E... New York:: Academic Press;.
    [Google Scholar]
  11. Kumar S., Nei M., Dudley J., Tamura K.. ( 2008;). mega: a biologist-centric software for evolutionary analysis of DNA and protein sequences. . Brief Bioinform 9:, 299–306. [CrossRef][PubMed]
    [Google Scholar]
  12. Lechevalier H., Lechevalier M. P.. ( 1965;). Classification des actinomycètes aérobies basée sur leur morphologie et leur composition chimique. . Ann Inst Pasteur (Paris) 108:, 662–673 (in French).[PubMed]
    [Google Scholar]
  13. Lechevalier M. P., Lechevalier H. A.. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (SIM Special Publication no. 6), pp. 227–291. Edited by Dietz A., Thayer D. W... Fairfax, VA:: Society for Industrial Microbiology;.
    [Google Scholar]
  14. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  15. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  16. Meier A., Kirschner P., Schröder K. H., Wolters J., Kroppenstedt R. M., Böttger E. C.. ( 1993;). Mycobacterium intermedium sp. nov.. Int J Syst Bacteriol 43:, 204–209. [CrossRef][PubMed]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H.. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  18. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Norcardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef][PubMed]
    [Google Scholar]
  19. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Shirling E. B., Gottlieb D.. ( 1966;). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16:, 313–340. [CrossRef]
    [Google Scholar]
  21. Stackebrandt E., Rainey F. A., Ward-Rainey N. L.. ( 1997;). Proposal for a new hierarchic classification system, Actinobacteria classis nov.. Int J Syst Bacteriol 47:, 479–491. [CrossRef]
    [Google Scholar]
  22. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  23. Waksman S. A.. ( 1961;). The Actinomycetes, vol. 2. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  24. Xu P., Li W.-J., Tang S.-K., Zhang Y.-Q., Chen G.-Z., Chen H.-H., Xu L.-H., Jiang C.-L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
  25. Yuan L.-J., Zhang Y.-Q., Guan Y., Wei Y.-Z., Li Q.-P., Yu L.-Y., Li W.-J., Zhang Y.-Q.. ( 2008;). Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. . Int J Syst Evol Microbiol 58:, 1180–1185. [CrossRef][PubMed]
    [Google Scholar]
  26. Zhang Y.-Q., Liu H.-Y., Yu L.-Y., Lee J. C., Park D. J., Kim C. J., Xu L.-H., Jiang C.-L., Li W.-J.. ( 2011;). Sinosporangium album gen. nov., sp. nov., a new member of the suborder Streptosporangineae. . Int J Syst Evol Microbiol 61:, 592–597. [CrossRef][PubMed]
    [Google Scholar]
  27. Zhi X.-Y., Li W.-J., Stackebrandt E.. ( 2009;). An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and emended descriptions of the existing higher taxa. . Int J Syst Evol Microbiol 59:, 589–608. [CrossRef][PubMed]
    [Google Scholar]
  28. Zhou Z.-H., Liu Z.-H., Qian Y.-D., Kim S. B., Goodfellow M.. ( 1998;). Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. . Int J Syst Bacteriol 48:, 53–58. [CrossRef][PubMed]
    [Google Scholar]
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