1887

Abstract

An endophytic actinomycete, designated strain I10A-01259, was isolated from a surface-sterilized fruit of (Rupr.) Maxim., a medicinal plant, which was collected from a suburb of Beijing, China. Whole-cell hydrolysates of the isolate contained galactose and -diaminopimelic acid. The predominant phospholipids were phosphatidylglycerol and diphosphatidylglycerol; the menaquinones consisted mainly of MK-9, MK-11 and MK-12, with a minor amount of MK-10. The major fatty acids were iso-C, anteiso-C and iso-C. Comparative analysis of 16S rRNA gene sequences indicated that strain I10A-01259 was most closely related to S186 (93.2 % sequence similarity), YIM 90462 (93.0 %) and other strains of genera within the families and . On the phylogenetic tree based on 16S rRNA gene sequences, strain I10A-01259 fell within the radius of the suborder , in which the strain formed a distinct lineage next to the genera of the families and . Based on the data from our polyphasic taxonomic study, a novel genus and species, gen. nov., sp. nov., are proposed within the suborder . The type strain of is strain I10A-01259 ( = CPCC 203428  = DSM 45601  = KCTC 19844).

Funding
This study was supported by the:
  • , National Infrastructure of Microbial Resources
  • , National Natural Science Foundation of China , (Award 81173026, 30870026 and 30970008)
  • , National S&T Major Special Project on Major New Drug Innovation , (Award 2010ZX09403, 2010ZX09402 and 2010ZX09401)
  • , Special Fund for Health-scientific Research in the Public Interest , (Award 201002021)
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2013-03-01
2020-07-15
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