1887

Abstract

A borrelidin-producing actinomycete, designated strain NEAU-W2, was isolated from the root surface of soybean [ (L.) Merr] and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. The G+C content of the DNA was 66.12 mol%. Analysis of the 16S rRNA gene sequence of strain NEAU-W2 revealed that the strain formed a distinct clade within the 16S rRNA gene sequence phylogenetic tree and showed highest similarity (99.61 %) to ATCC 27449. However, the DNA–DNA relatedness between strain NEAU-W2 and ATCC 27449 was 58.51 %. Strain NEAU-W2 could also be differentiated from ATCC 27449 and other species showing high 16S rRNA gene sequence similarity (98–99 %), as well as other borrelidin-producing strains, based on morphological and physiological characteristics. On the basis of its physiological and molecular properties, it is proposed that strain NEAU-W2 represents a novel species, sp. nov. The type strain is NEAU-W2 ( = CGMCC 4.7004  = ATCC BAA-2424  = DSM 42073).

Funding
This study was supported by the:
  • , NKPBR , (Award 2010CB126102)
  • , NNSF , (Award 30771427 and 30971937)
  • , Special Foundation for Scientific and Technological Innovation Research of Harbin , (Award 2011RFXXN038)
  • , Graduate Innovation Scientific Research Project of Heilongjiang Province , (Award YJSCX2011-066HLJ)
  • , NSF , (Award C201029)
  • , NIH , (Award GM086184)
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2013-03-01
2020-08-08
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