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Volume 63, Issue Pt_5

sp. nov., isolated from the red alga , and emended descriptions of the genus and two species Free

Abstract

Three Gram-negative, non-motile, strictly aerobic strains, designated LNM-20, LCM-1 and LAM-13, were isolated from thalli of the marine red alga . Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates were associated with the genus in the family and were most closely related to DSW-5 (96.2 % 16S rRNA gene sequence similarity) and K17-16 (95.0 %). The DNA G+C content of the isolates was 28.6–29.2 mol%. DNA–DNA hybridization analysis showed that the isolates belonged to a single species distinct from both of their closest relatives. The only isoprenoid quinone detected was menaquinone-6. The main polar lipids were phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids. The major fatty acids were iso-C, iso-Cω10 and iso-C 3-OH. The phenotypic features of strain LNM-20 differed from those of their closest relatives in several regards (colony colour, growth with 1 % NaCl and on TSA plus 2.5 % NaCl, hydrolysis of Tweens 40 and 80, and oxidization of five carbon compounds). On the basis of phylogenetic, chemotaxonomic and phenotypic analysis, the isolates represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain is LNM-20 ( = LMG 26671  = NBRC 108759). Emended descriptions of the genus and and are also proposed.

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Funding
This study was supported by the:
  • Japan Society for the Promotion of Science for Young Scientists (Award 23.4176)
© 2013 IUMS
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2013-05-01
2024-04-18
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References

  1. Akagawa-Matsushita M., Itoh T., Katayama Y., Kuraishi H., Yamasato K. ( 1992 ). Isoprenoid quinone composition of some marine Alteromonas, Marinomonas, Deleya, Pseudomonas and Shewanella species. . J Gen Microbiol 138, 2275–2281. [CrossRef]
    [Google Scholar]
  2. Alain K., Tindall B. J., Intertaglia L., Catala P., Lebaron P. ( 2008 ). Hellea balneolensis gen. nov., sp. nov., a prosthecate alphaproteobacterium from the Mediterranean Sea. . Int J Syst Evol Microbiol 58, 2511–2519. [View Article] [PubMed]
    [Google Scholar]
  3. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403–410.[PubMed] [CrossRef]
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  5. Bowman J. P. ( 2000 ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50, 1861–1868.[PubMed]
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 1997–2006. [View Article] [PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  8. Felsenstein J. ( 2009 ). phylip (phylogeny inference package), version 3.69. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Gosink J. J., Woese C. R., Staley J. T. ( 1998 ). Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroides group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov.. Int J Syst Bacteriol 48, 223–235. [View Article] [PubMed]
    [Google Scholar]
  10. Ikemoto S., Katoh K., Komagata K. ( 1978 ). Cellular fatty acid composition in methanol-utilizing bacteria. . J Gen Appl Microbiol 24, 41–49. [View Article]
    [Google Scholar]
  11. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  13. Lee Y. S., Lee D.-H., Kahng H.-Y., Sohn S. H., Jung J. S. ( 2011 ). Polaribacter gangjinensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 1425–1429. [View Article] [PubMed]
    [Google Scholar]
  14. Luthria D. L., Sprecher H. ( 1993 ). 2-Alkenyl-4,4-dimethyloxazolines as derivatives for the structural elucidation of isomeric unsaturated fatty acids. . Lipids 28, 561–564. [View Article] [PubMed]
    [Google Scholar]
  15. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  16. McGuire A. J., Franzmann P. D., McMeekin T. A. ( 1987 ). Flectobacillus glomeratus sp. nov., a curved, nonmotile, pigmented bacterium isolated from Antarctic marine environments. . Syst Appl Microbiol 9, 265–272. [View Article]
    [Google Scholar]
  17. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  18. Nedashkovskaya O. I., Kim S. B., Lysenko A. M., Kalinovskaya N. I., Mikhailov V. V., Kim I. S., Bae K. S. ( 2005 ). Polaribacter butkevichii sp. nov., a novel marine mesophilic bacterium of the family Flavobacteriaceae . . Curr Microbiol 51, 408–412. [View Article] [PubMed]
    [Google Scholar]
  19. Ogata E. ( 1970 ). [On a new algal culture medium SWM-III]. . Bull Jpn Soc Phycol 18, 171–173 (in Japanese).
     [Google Scholar]
  20. Satomi M., Kimura B., Mizoi M., Sato T., Fujii T. ( 1997 ). Tetragenococcus muriaticus sp. nov., a new moderately halophilic lactic acid bacterium isolated from fermented squid liver sauce. . Int J Syst Bacteriol 47, 832–836. [View Article] [PubMed]
    [Google Scholar]
  21. Sawabe T., Oda Y., Shiomi Y., Ezura Y. ( 1995 ). Alginate degradation by bacteria isolated from the gut of sea urchins and abalones. . Microb Ecol 30, 193–202. [View Article]
    [Google Scholar]
  22. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  23. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 1596–1599. [View Article] [PubMed]
    [Google Scholar]
  25. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  27. Yoon J.-H., Kang S.-J., Oh T.-K. ( 2006 ). Polaribacter dokdonensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 56, 1251–1255. [View Article] [PubMed]
    [Google Scholar]
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Polaribacter porphyrae sp. nov., isolated from the red alga Porphyra yezoensis, and emended descriptions of the genus Polaribacter and two Polaribacter species
Int J Syst Evol Microbiol 63, 1665 (2013); https://doi.org/10.1099/ijs.0.041434-0
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