1887

Abstract

A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain E103, was isolated from the skin of the medical leech . 16S rRNA gene sequence analysis showed that the isolate was closely related to species of the genus DCY36 was shown to be the most closely related (98.4 % 16S rRNA gene sequence similarity), followed by NKNTAU and MJ06 (both 97.8 %), then Ho-11 (97.5 %). Chemotaxonomic data (major ubiquinone, Q-8; major polar lipids, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine; predominant polyamine, putrescine with a moderate amount of 2-hydroxyputrescine; and major fatty acids, C cyclo, C and summed feature 4 comprising Cω7 and/or iso-C 2-OH) supported the affiliation of the isolate to the genus . DNA–DNA hybridization values with the type strains of all species of the genus were 23 % (reciprocal, 18 %) with KCTC 22398, 20 % (26 %) with KCTC 22454, 11 % (58 %) with DSM 11046 and 13 % (12 %) with KCTC 12197 Phenotypic differentiation of strain E103 from its closest neighbours was possible. Strain E103 therefore represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain E103 ( = CCUG 62394 = LMG 26910).

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2013-02-01
2021-10-23
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