A bacterial strain designated M1MS02T was isolated from a surface-sterilized nodule of Medicago sativa in Zamora (Spain). The 16S rRNA gene sequence of this strain showed 96.5 and 96.2 % similarity, respectively, with respect to Gluconacetobacter liquefaciens IFO 12388T and Granulibacter bethesdensis CGDNIH1T from the family Acetobacteraceae. The novel isolate was a Gram-stain-negative, non-sporulating, aerobic coccoid to rod-shaped bacterium that was motile by a subpolar flagellum. The major fatty acid was C18 : 1ω7c and the major ubiquinone was Q-10. The lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, three aminolipids, four glycolipids, two phospholipids and one lipid. Strain M1MS02T was catalase-positive and oxidase- and urease-negative. Acetate and lactate were not oxidized. Acetic acid was produced from ethanol in culture media supplemented with 2 % CaCO3. Ammonium sulphate was assimilated in glucose medium. The strain produced dihydroxyacetone from glycerol. Phylogenetic and phenotypic analyses commonly used to differentiate genera within the family Acetobacteraceae showed that strain M1MS02T should be classified as representing a novel species of a new genus within this family, for which the name Endobacter medicaginis gen. nov., sp. nov. is proposed. The type strain of the type species is M1MS02T ( = LMG 26838T = CECT 8088T). To our knowledge, this is the first report of a member of the Acetobacteraceae occurring as a legume nodule endophyte.
ChunJ.,
GoodfellowM.(1995). A phylogenetic analysis of the genus Nocardia with 16S rRNA sequences. . Int J Syst Bacteriol45, 240–245. [View Article][PubMed]
DjedidiS.,
YokoyamaT.,
Ohkama-OhtsuN.,
RisalC. P.,
AbdellyC.,
SekimotoH.(2011). Stress tolerance and symbiotic and phylogenic features of root nodule bacteria associated with Medicago species in different bioclimatic regions of Tunisia. . Microbes Environ26, 36–45. [View Article][PubMed]
DoetschR. N.(1981). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 21–33. Edited by
GerdhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.
. Washington, DC:: American Society for Microbiology;.
DongZ.,
CannyM. J.,
McCullyM. E.,
RoboredoM. R.,
CabadillaC. F.,
OrtegaE.,
RodésR.(1994). A nitrogen-fixing endophyte of sugarcane. A new role for the apoplast. . Plant Physiol105, 1139–1147.[PubMed]
GauntM. W.,
TurnerS. L.,
Rigottier-GoisL.,
Lloyd-MacgilpS. A.,
YoungJ. P.(2001). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. . Int J Syst Evol Microbiol51, 2037–2048. [View Article][PubMed]
GreenbergD. E.,
PorcellaS. F.,
StockF.,
WongA.,
ConvilleP. S.,
MurrayP. R.,
HollandS. M.,
ZelaznyA. M.(2006).Granulibacter bethesdensis gen. nov., sp. nov., a distinctive pathogenic acetic acid bacterium in the family Acetobacteraceae
. . Int J Syst Evol Microbiol56, 2609–2616. [View Article][PubMed]
KanF. L.,
ChenZ. Y.,
WangE. T.,
TianC. F.,
SuiX. H.,
ChenW. X.(2007). Characterization of symbiotic and endophytic bacteria isolated from root nodules of herbaceous legumes grown in Qinghai-Tibet plateau and in other zones of China. . Arch Microbiol188, 103–115. [View Article][PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
MandelM.,
MarmurJ.(1968). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol12, 195–206. [View Article]
RivasR.,
García-FraileP.,
MateosP. F.,
Martínez-MolinaE.,
VelázquezE.(2007). Characterization of xylanolytic bacteria present in the bract phyllosphere of the date palm Phoenix dactylifera
. . Lett Appl Microbiol44, 181–187. [View Article][PubMed]
RogersJ. S.,
SwoffordD. L.(1998). A fast method for approximating maximum likelihoods of phylogenetic trees from nucleotide sequences. . Syst Biol47, 77–89. [View Article][PubMed]
SaravananV. S.,
MadhaiyanM.,
OsborneJ.,
ThangarajuM.,
SaT. M.(2008). Ecological occurrence of Gluconacetobacter diazotrophicus and nitrogen-fixing Acetobacteraceae members: their possible role in plant growth promotion. . Microb Ecol55, 130–140. [View Article][PubMed]
SieversM.,
SwingsJ.(2005). Family II: Acetobacteraceae Gillis and de Ley 1980, 23VP
. . In Bergey’s Manual of Systematic Bacteriology, vol. 2, pp. 41–95. Edited by
BrennerD. J.,
KriegN. R.,
StanleyJ. T.
. New York:: Springer;.
TindallB. J.(1990a). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol13, 128–130. [View Article]
TrujilloM. E.,
Alonso-VegaP.,
RodríguezR.,
CarroL.,
CerdaE.,
AlonsoP.,
Martínez-MolinaE.(2010). The genus Micromonospora is widespread in legume root nodules: the example of Lupinus angustifolius
. . ISME J4, 1265–1281. [View Article][PubMed]
VincentJ. M.(1970). The cultivation, isolation and maintenance of rhizobia. . In A Manual for the Practical Study of Root-Nodule, pp. 1–13. Edited by
VincentJ. M.
. Oxford:: Blackwell Scientific Publications;.
YamashitaS.,
UchimuraT.,
KomagataK.(2004). Emendation of the genus Acidomonas Urakami, Tamaoka, Suzuki and Komagata 1989. . Int J Syst Evol Microbiol54, 865–870. [View Article][PubMed]