1887

Abstract

A novel Gram-stain-positive, moderately halophilic bacterium, designated strain G8B, was isolated from water of the hypersaline lake Aran-Bidgol in Iran and characterized taxonomically using a polyphasic approach. Cells of strain G8B were rod-shaped, motile and produced oval endospores at a terminal position in swollen sporangia. Strain G8B was strictly aerobic, catalase-positive and oxidase-negative. The strain was able to grow at NaCl concentrations of 0.5–12.5 % (w/v), with optimum growth occurring at 5–7.5 % (w/v) NaCl. The optimum temperature and pH for growth were 35–40 °C and pH 7.5–8.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain G8B was shown to belong to the genus within the phylum and showed closest phylogenetic similarity with WSBC 24001 (97.6 %). The DNA G+C content of strain G8B was 36.9 mol%. The major cellular fatty acids of strain G8B were anteiso-C, anteiso-C, iso-C and iso-C, and its polar lipid pattern consisted of phosphatidylglycerol, diphosphatidylglycerol, four unknown phospholipids and an unknown aminolipid. The isoprenoid quinones were MK-7 (98 %) and MK-8 (2 %). Strain G8B contained a peptidoglycan of type A4β, -Orn–-Asp. All these features confirmed the placement of isolate G8B within the genus . DNA–DNA hybridization experiments revealed a low level of relatedness (6 %) between strain G8B and DSM 15681. On the basis of evidence from this study, a novel species of the genus , sp. nov., is proposed, with strain G8B ( = IBRC-M 10683 = KCTC 13822) as the type strain.

Funding
This study was supported by the:
  • Iranian Biological Resource Centre (IBRC) (Award MI-1388-04)
  • Spanish Ministerio de Economía y Competitividad (Award CGL2010-19303)
  • National Science Foundation (Award Grant DEB-0919290)
  • Junta de Andalucía (Award P10-CVI-6226)
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2013-03-01
2024-03-28
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References

  1. Baron E. J., Finegold S. M. ( 1990 ). Bailey and Scott’s Diagnostic Microbiology, , 8th edn.. St Louis:: Mosby;.
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  5. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406416. [View Article]
    [Google Scholar]
  6. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [View Article] [PubMed]
    [Google Scholar]
  7. Harrigan W. F., McCance M. E. ( 1976 ). Laboratory Methods in Food and Dairy Microbiology. London:: Academic Press;.
    [Google Scholar]
  8. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article]
    [Google Scholar]
  9. Kämpfer P., Falsen E., Lodders N., Langer S., Busse H.-J., Schumann P. ( 2010 ). Ornithinibacillus contaminans sp. nov., an endospore-forming species. . Int J Syst Evol Microbiol 60, 29302934. [View Article] [PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  12. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). ARB: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  13. MacKenzie S. L. ( 1987 ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. . J Assoc Off Anal Chem 70, 151160.[PubMed]
    [Google Scholar]
  14. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  15. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. ( 2002 ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25, 360375. [View Article] [PubMed]
    [Google Scholar]
  16. Mayr R., Busse H.-J., Worliczek H. L., Ehling-Schulz M., Scherer S. ( 2006 ). Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov.. Int J Syst Evol Microbiol 56, 13831389. [View Article] [PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  18. Monciardini P., Cavaletti L., Schumann P., Rohde M., Donadio S. ( 2003 ). Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria . . Int J Syst Evol Microbiol 53, 569576. [View Article] [PubMed]
    [Google Scholar]
  19. Murray R. G. E., Doetsch R. N., Robinow C. F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1984 ). Deleya halophila, a new species of moderately halophilic bacteria. . Int J Syst Bacteriol 34, 287292. [View Article]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123156. [View Article]
    [Google Scholar]
  23. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982 ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128, 19591968.
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
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