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Volume 63, Issue Pt_2

gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring Free

Abstract

A novel thermophilic bacterium, strain Kam1851, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851 were spore-forming rods with a Gram-positive type of cell wall. Growth was observed between 46 and 78 °C, and pH 5.5–8.5. The optimal growth (doubling time, 6.0 h) was at 60–65 °C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C (34.2 %), iso-C (18 %), C (12.8 %) and iso-C (11.1 %). The G+C content of the genomic DNA of strain Kam1851 was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851 belonged to the order , but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2 % sequence similarity, were members of the genera and . On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851 is proposed to represent a novel species in a new genus, gen. nov., sp. nov.; the type strain of is Kam1851 ( = DSM 22653 = VKM B-2685).

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Funding
This study was supported by the:
  • Federal Ministry of Education and Science program ‘Kadry’ (Award П646 and П2283)
  • Russian Academy of Sciences programs ‘Molecular and Cell Biology’ and ‘Origin and Evolution of the Biosphere’
© 2013 IUMS
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2013-02-01
2024-04-19
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Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring
Int J Syst Evol Microbiol 63, 479 (2013); https://doi.org/10.1099/ijs.0.041285-0
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