1887

Abstract

A novel, Gram-staining-negative, non-pigmented, rod-shaped, strictly aerobic, extremely halophilic bacterium, designated strain IA16, was isolated from the mud of the hypersaline Lake Aran-Bidgol, in Iran. Cells of strain IA16 were not motile. Growth occurred with 2.5–5.2 M NaCl (optimum 3.4 M), at pH 6.0–8.0 (optimum pH 7.0) and at 30–50 °C (optimum 40 °C). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain IA16 belonged in the family and that its closest relatives were DSM 9895 (91.6 % sequence similarity), NCIMB 2243 (91.2 %), CL-UU02 (88.9 %) and YIM D82 (88.7 %). The novel strain’s major cellular fatty acids were C cyclo ω7 and C and its polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, four unidentified phospholipids, three unidentified aminolipids and two other unidentified lipids. The cells of strain IA16 contained the ubiquinone Q-10. The G+C content of the novel strain’s genomic DNA was 67.0 mol%. The physiological, biochemical and phylogenetic differences between strain IA16 and other previously described taxa indicate that the strain represents a novel species in a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is IA16 ( = IBRC-M 10018  = DSM 25584).

Funding
This study was supported by the:
  • Iranian Biological Resource Centre (Award MI-1388-01)
  • International Foundation for Science (Award A/4527-1)
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2013-04-01
2024-12-08
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