1887

Abstract

A set of 25 urease-producing, yellow-pigmented enterococci was isolated from environmental sources. Phenotypic classification divided the isolates into two phena. Both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all members of the genus with validly published names and placed them in the species group. DNA–DNA hybridization experiments, and sequencing and whole-cell protein electrophoresis provided conclusive evidence for the classification of each phenon as a novel species of the genus , for which the names sp. nov. (type strain CCM 4629  = LMG 26676  = CCUG 48799), inhabiting water and plants, and sp. nov. (type strain CCM 4630  = LMG 26678  = CCUG 61593), inhabiting water, insects (mosquitoes) and plants, are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.041152-0
2013-02-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/2/502.html?itemId=/content/journal/ijsem/10.1099/ijs.0.041152-0&mimeType=html&fmt=ahah

References

  1. Brisse S., Fussing V., Ridwan B., Verhoef J., Willems R. J. L.. ( 2002;). Automated ribotyping of vancomycin-resistant Enterococcus faecium isolates. . J Clin Microbiol 40:, 1977–1984. [CrossRef][PubMed]
    [Google Scholar]
  2. Devriese L. A., Collins M. D., Wirth R.. ( 1992;). The genus Enterococcus. . In The Prokaryotes: a handbook on the biology of bacteria, , 2nd edn., pp. 1465–1481. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H... New York:: Springer-Verlag;.
    [Google Scholar]
  3. Euzéby J. P.. ( 1997;). List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47:, 590–592. [CrossRef][PubMed]
    [Google Scholar]
  4. Facklam R. R., Collins M. D.. ( 1989;). Identification of Enterococcus species isolated from human infections by a conventional test scheme. . J Clin Microbiol 27:, 731–734.[PubMed]
    [Google Scholar]
  5. Facklam R. R., Washington J. A. II. ( 1991;). Streptococcus and related catalase-negative gram-positive cocci. . In Manual of Clinical Microbiology, , 5th edn., pp. 238–257. Edited by Balows A., Hausler W. J., Herrmann K. L., Isenberg H. D., Shadomy H. J... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  6. Facklam R. R., Hollis D., Collins M. D.. ( 1989;). Identification of gram-positive coccal and coccobacillary vancomycin-resistant bacteria. . J Clin Microbiol 27:, 724–730.[PubMed]
    [Google Scholar]
  7. Facklam R. R., Sahm D. F., Teixeira L. M.. ( 1999;). Enterococcus. . In Manual of Clinical Microbiology, , 7th edn., pp. 297–305. Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  9. Fisher K., Phillips C.. ( 2009;). The ecology, epidemiology and virulence of Enterococcus. . Microbiology 155:, 1749–1757. [CrossRef][PubMed]
    [Google Scholar]
  10. Foulquié Moreno M. R., Sarantinopoulos P., Tsakalidou E., De Vuyst L.. ( 2006;). The role and application of enterococci in food and health. . Int J Food Microbiol 106:, 1–24. [CrossRef][PubMed]
    [Google Scholar]
  11. Franz C. M. A. P., Stiles M. E., Schleifer K. H., Holzapfel W. H.. ( 2003;). Enterococci in foods – a conundrum for food safety. . Int J Food Microbiol 88:, 105–122. [CrossRef][PubMed]
    [Google Scholar]
  12. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K.. ( 1998;). Evaluation of a microplate DNA–DNA hybridization method compared with the initial renaturation method. . Can J Microbiol 44:, 1148–1153. [CrossRef]
    [Google Scholar]
  13. Hartel P. G., Myoda S. P., Ritter K. J., Kuntz R. L., Rodgers K., Entry J. A., Ver Wey S. A., Schröder E. C., Calle J.. & other authors ( 2007;). Geographic sharing of ribotype patterns in Enterococcus faecalis for bacterial source tracking. . J Water Health 5:, 539–551. [CrossRef][PubMed]
    [Google Scholar]
  14. Hugas M., Garriga M., Aymerich M. T.. ( 2003;). Functionality of enterococci in meat products. . Int J Food Microbiol 88:, 223–233. [CrossRef][PubMed]
    [Google Scholar]
  15. Jett B. D., Huycke M. M., Gilmore M. S.. ( 1994;). Virulence of enterococci. . Clin Microbiol Rev 7:, 462–478.[PubMed]
    [Google Scholar]
  16. Kühn I., Burman L. G., Haeggman S., Tullus K., Murray B. E.. ( 1995;). Biochemical fingerprinting compared with ribotyping and pulsed-field gel electrophoresis of DNA for epidemiological typing of enterococci. . J Clin Microbiol 33:, 2812–2817.[PubMed]
    [Google Scholar]
  17. Kühn I., Iversen A., Burman L. G., Olsson-Liljequist B., Franklin A., Finn M., Aarestrup F., Seyfarth A. M., Blanch A. R.. & other authors ( 2003;). Comparison of enterococcal populations in animals, humans, and the environment – a European study. . Int J Food Microbiol 88:, 133–145. [CrossRef][PubMed]
    [Google Scholar]
  18. Kuntz R. L., Hartel P. G., Godfrey D. G., McDonald J. L., Gates K. W., Segars W. I.. ( 2003;). Targeted sampling protocol as prelude to bacterial source tracking with Enterococcus faecalis. . J Environ Qual 32:, 2311–2318. [CrossRef][PubMed]
    [Google Scholar]
  19. Merquior V. L. C., Peralta J. M., Facklam R. R., Teixeira L. M.. ( 1994;). Analysis of electrophoretic whole-cell protein profiles as a tool for characterisation of Enterococcus species. . Curr Microbiol 28:, 149–153. [CrossRef]
    [Google Scholar]
  20. Müller T., Ulrich A., Ott E.-M., Müller M.. ( 2001;). Identification of plant-associated enterococci. . J Appl Microbiol 91:, 268–278. [CrossRef][PubMed]
    [Google Scholar]
  21. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J.. ( 2005;). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. . Microbiology 151:, 2141–2150. [CrossRef][PubMed]
    [Google Scholar]
  22. NCCLS ( 2003;). Performance standards for antimicrobial disk susceptibility. 8th edition. Approved standard M2–A8. NCCLS, Wayne, Pa.
  23. Niemi R. M., Ollinkangas T., Paulin L., Švec P., Vandamme P., Karkman A., Kosina M., Lindström K.. ( 2012;). Enterococcus rivorum sp. nov., from water of pristine brooks. . Int J Syst Evol Microbiol 62:, 2169–2173. [CrossRef][PubMed]
    [Google Scholar]
  24. Pot B., Vandamme P., Kersters K.. ( 1994;). Analysis of electrophoretic whole-organism protein fingerprints. . In Modern Microbiological Methods: Chemical Methods in Prokaryotic Systematics, pp. 493–521. Edited by Goodfellow M., O’Donnell A. G... Chichester, UK:: John Wiley & Sons Ltd;.
    [Google Scholar]
  25. Pryce T. M., Wilson R. D., Kulski J. K.. ( 1999;). Identification of enterococci by ribotyping with horseradish-peroxidase-labelled 16S rDNA probes. . J Microbiol Methods 36:, 147–155. [CrossRef][PubMed]
    [Google Scholar]
  26. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E.. ( 1996;). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46:, 1088–1092. [CrossRef][PubMed]
    [Google Scholar]
  27. Rice L. B.. ( 2001;). Emergence of vancomycin-resistant enterococci. . Emerg Infect Dis 7:, 183–187. [CrossRef][PubMed]
    [Google Scholar]
  28. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  29. Sistek V., Maheux A. F., Boissinot M., Bernard K. A., Cantin P., Cleenwerck I., De Vos P., Bergeron M. G.. ( 2012;). Enterococcus ureasiticus sp. nov. and Enterococcus quebecensis sp. nov., isolated from water. . Int J Syst Evol Microbiol 62:, 1314–1320. [CrossRef][PubMed]
    [Google Scholar]
  30. Sperber W. H., Swan J.. ( 1976;). Hot-loop test for the determination of carbon dioxide production from glucose by lactic acid bacteria. . Appl Environ Microbiol 31:, 990–991.[PubMed]
    [Google Scholar]
  31. Švec P., Devriese L. A.. ( 2009;). Genus I. Enterococcus. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, pp. 594–607. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K.-H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  32. Švec P., Devriese L. A., Sedlácek I., Baele M., Vancanneyt M., Haesebrouck F., Swings J., Doškař J.. ( 2001a;). Enterococcus haemoperoxidus sp. nov. and Enterococcus moraviensis sp. nov., isolated from water. . Int J Syst Evol Microbiol 51:, 1567–1574.[PubMed]
    [Google Scholar]
  33. Švec P., Sedlácek I., Pantůcek R., Devriese L. A., Doškař J. V.. ( 2001b;). Evaluation of ribotyping for characterization and identification of Enterococcus haemoperoxidus and Enterococcus moraviensis strains. . FEMS Microbiol Lett 203:, 23–27. [CrossRef][PubMed]
    [Google Scholar]
  34. Švec P., Vancanneyt M., Seman M., Snauwaert C., Lefebvre K., Sedlácek I., Swings J.. ( 2005;). Evaluation of (GTG)5-PCR for identification of Enterococcus spp.. FEMS Microbiol Lett 247:, 59–63. [CrossRef][PubMed]
    [Google Scholar]
  35. Švec P., Nováková D., Zácková L., Kukletová M., Sedlácek I.. ( 2008;). Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans. . Antonie van Leeuwenhoek 94:, 573–579. [CrossRef][PubMed]
    [Google Scholar]
  36. Švec P., Vandamme P. A., Bryndová H., Holochová P., Kosina M., Maslanová I., Sedlácek I.. ( 2012;). Enterococcus plantarum sp. nov., isolated from plants. . Int J Syst Evol Microbiol 62:, 1499–1505. [CrossRef][PubMed]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  38. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  39. Vancanneyt M., Zamfir M., Devriese L. A., Lefebvre K., Engelbeen K., Vandemeulebroecke K., Amar M., De Vuyst L., Haesebrouck F., Swings J.. ( 2004;). Enterococcus saccharominimus sp. nov., from dairy products. . Int J Syst Evol Microbiol 54:, 2175–2179. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.041152-0
Loading
/content/journal/ijsem/10.1099/ijs.0.041152-0
Loading

Data & Media loading...

Supplements

Supplementary material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error