@article{mbs:/content/journal/ijsem/10.1099/ijs.0.041111-0, author = "Zou, Yuanqiang and Liu, Feng and Fang, Chengxiang and Wan, Daiwei and Yang, Rentao and Su, Qingqing and Yang, Ruifu and Zhao, Jiao", title = "Lactobacillus shenzhenensis sp. nov., isolated from a fermented dairy beverage", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2013", volume = "63", number = "Pt_5", pages = "1817-1823", doi = "https://doi.org/10.1099/ijs.0.041111-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.041111-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two Lactobacillus strains, designated LY-73T and LY-30B, were isolated from a dairy beverage, sold in Shenzhen market, China. The two isolates were Gram-positive, non-spore-forming, non-motile, facultatively anaerobic rods that were heterofermentative and did not exhibit catalase activity. Sequencing of the 16S rRNA, pheS and rpoA genes revealed that the two isolates shared 99.5, 99.8 and 99.9 % sequence similarity, which indicates that they belong to the same species. Phylogenetic analysis demonstrated clustering of the two isolates with the genus Lactobacillus . Strain LY-73T showed highest 16S rRNA gene sequence similarities with Lactobacillus harbinensis KACC 12409T (97.73 %), Lactobacillus perolens DSM 12744T (96.96 %) and Lactobacillus selangorensis DSM 13344T (93.10 %). Comparative analyses of their rpoA and pheS gene sequences indicated that the novel strains were significantly different from other Lactobacillus species. Low DNA–DNA reassociation values (50.5 %) were obtained between strain LY-73T and its phylogenetically closest neighbours. The G+C contents of the DNA of the two novel isolates were 56.1 and 56.5 mol%. Straight-chain unsaturated fatty acids C18 : 1ω9c (78.85 and 74.29 %) were the dominant components, and the cell-wall peptidoglycan was of the l-Lys–d-Asp type. Based on phenotypic characteristics, and chemotaxonomic and genotypic data, the novel strains represent a novel species of the genus Lactobacillus , for which the name Lactobacillus shenzhenensis sp. nov. is proposed, with LY-73T ( = CCTCC M 2011481T = KACC 16878T) as the type strain.", }