1887

Abstract

Two strains, designated LY-73 and LY-30B, were isolated from a dairy beverage, sold in Shenzhen market, China. The two isolates were Gram-positive, non-spore-forming, non-motile, facultatively anaerobic rods that were heterofermentative and did not exhibit catalase activity. Sequencing of the 16S rRNA, and genes revealed that the two isolates shared 99.5, 99.8 and 99.9 % sequence similarity, which indicates that they belong to the same species. Phylogenetic analysis demonstrated clustering of the two isolates with the genus . Strain LY-73 showed highest 16S rRNA gene sequence similarities with KACC 12409 (97.73 %), DSM 12744 (96.96 %) and DSM 13344 (93.10 %). Comparative analyses of their and gene sequences indicated that the novel strains were significantly different from other species. Low DNA–DNA reassociation values (50.5 %) were obtained between strain LY-73 and its phylogenetically closest neighbours. The G+C contents of the DNA of the two novel isolates were 56.1 and 56.5 mol%. Straight-chain unsaturated fatty acids Cω9 (78.85 and 74.29 %) were the dominant components, and the cell-wall peptidoglycan was of the -Lys–-Asp type. Based on phenotypic characteristics, and chemotaxonomic and genotypic data, the novel strains represent a novel species of the genus , for which the name sp. nov. is proposed, with LY-73 ( = CCTCC M 2011481 = KACC 16878) as the type strain.

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2013-05-01
2020-01-23
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