Four orange-pigmented isolates, L7-456, L7-484T, L9-479 and L9-753T, originating from surface-sterilized leaf tissues of Jatropha curcas L. cultivars were characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that all four isolates belong to the genus Aureimonas. In these analyses, strain L7-484T appeared to be most closely related to Aureimonas ureilytica 5715S-12T (95.7 % sequence identity). The 16S rRNA gene sequences of strains L7-456, L9-479 and L9-753T were found to be identical and also shared the highest similarity with A. ureilytica 5715S-12T (97.5 %). Both L7-484T and L9-753T contained Q-10 and Q-9 as predominant ubiquinones and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, sulfoquinovosyldiacylglycerol and an aminophospholipid as the major polar lipids. C18 : 1ω7c and C16 : 0 were the major fatty acids. Similar to other species in the genus Aureimonas, hydroxylated fatty acids (e.g. C18 : 1 2-OH) and cyclic fatty acids (C19 : 0 cyclo ω8c) were also present. The DNA G+C contents of L7-484T and L9-753T were 66.1 and 69.4 mol%, respectively. Strains L7-484T and L9-753T exhibited less than 40 % DNA–DNA hybridization both between themselves and to A. ureilytica KACC 11607T. Our results support the proposal that strain L7-484T represents a novel species within the genus Aureimonas, for which the name Aureimonas jatrophae sp. nov. is proposed, and that strains L9-753T, L7-456 ( = KACC 16229 = DSM 25023) and L9-479 ( = KACC 16228 = DSM 25024) represent a second novel species within the genus, for which the name Aureimonas phyllosphaerae sp. nov. is proposed. The type strains of Aureimonas jatrophae sp. nov. and Aureimonas phyllosphaerae sp. nov. are respectively L7-484T ( = KACC 16230T = DSM 25025T) and L9-753T ( = KACC 16231T = DSM 25026T).
IkedaS.,
OkuboT.,
KanekoT.,
InabaS.,
MaekawaT.,
EdaS.,
SatoS.,
TabataS.,
MitsuiH.,
MinamisawaK.(2010a). Community shifts of soybean stem-associated bacteria responding to different nodulation phenotypes and N levels. . ISME J4, 315–326. [View Article][PubMed]
IkedaS.,
AndaM.,
InabaS.,
EdaS.,
SatoS.,
SasakiK.,
TabataS.,
MitsuiH.,
SatoT.& other authors (2011). Autoregulation of nodulation interferes with impacts of nitrogen fertilization levels on the leaf-associated bacterial community in soybeans. . Appl Environ Microbiol77, 1973–1980. [View Article][PubMed]
JuradoV.,
GonzalezJ. M.,
LaizL.,
Saiz-JimenezC.(2006).Aurantimonas altamirensis sp. nov., a member of the order Rhizobiales isolated from Altamira Cave. . Int J Syst Evol Microbiol56, 2583–2585. [View Article][PubMed]
MesbahM.,
PremachandranU.,
WhitmanW. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol39, 159–167. [View Article]
MinnikinD. E.,
O’DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalK.,
ParlettJ. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods2, 233–241. [View Article]
RathsackK.,
ReitnerJ.,
StackebrandtE.,
TindallB. J.(2011). Reclassification of Aurantimonas altamirensis (Jurado et al. 2006), Aurantimonas ureilytica (Weon et al. 2007) and Aurantimonas frigidaquae (Kim et al. 2008) as members of a new genus, Aureimonas gen. nov., as Aureimonas altamirensis gen. nov., comb. nov., Aureimonas ureilytica comb. nov. and Aureimonas frigidaquae comb. nov., and emended descriptions of the genera Aurantimonas and Fulvimarina
. . Int J Syst Evol Microbiol61, 2722–2728. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
WilsonK.(1987). Preparation of genomic DNA from bacteria. . In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.5. Edited by
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.
. New York:: Green Publishing & Wiley-Interscience;.