1887

Abstract

A novel, dissimilatory Fe(III)-reducing bacterium, designated strain SgZ-2, which could couple glucose oxidation to iron reduction for energy conservation, was isolated from a microbial fuel cell. The isolate was Gram-staining-negative, catalase-positive, oxidase-negative and facultatively anaerobic. The strain was able to grow on tryptic soy agar at 15–37 °C and in the presence of 0–5 % (w/v) NaCl. The predominant cellular fatty acids (>5 %) were iso-C, iso-C 3-OH and iso-Cω9; the major respiratory quinone was MK-7; the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and three other unidentified lipids; and the DNA G+C content was 55.3 mol%. Phylogenetic analyses based on 16S rRNA sequences showed that the novel strain was most closely related to CC-GZM-130 (99.9 % sequence similarity), and was a member of the family The levels of DNA–DNA relatedness observed between strain SgZ-2 and CCM 7650 (<41 %) indicated that the two strains represented two distinct species. Based on phylogenetic analyses and phenotypic characteristics, strain SgZ-2 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SgZ-2 ( = CCTCC M 2011498  = KACC 16525).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31070460, 21177030 and 41171205)
  • , Youth Foundation of the Guangdong Academy of Sciences , (Award Z113546)
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2013-03-01
2020-07-10
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