1887

Abstract

Two Gram-positive-staining, rod-shaped, endospore-forming isolates (UG-2 and UG-3), with an optimum growth temperature of around 37 °C and an optimum pH for growth of about 4, were recovered from an acidic effluent of the uranium mill tailing at Urgeiriça in Central Portugal. On the basis of 16S rRNA gene sequence similarity, the strains belonged to the family and were closely related to ATCC 53909 (97.9 %). Unlike , strains UG-2 and UG-3 grew in medium containing up to 5000 p.p.m. U(VI) but did not hydrolyse pullulan. Chemotaxonomic data also supported the affiliation of strains UG-2 and UG-3 to the genus . Physiological and biochemical tests along with fatty acid composition allowed differentiation of strains UG-2 and UG-3 from . It is suggested that strains UG-2 and UG-3 represent a novel species, for which the name is proposed; the type strain is UG-2 ( = DSM 19429 = LMG 24205). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award PTDC/BIA-MIC/105247/2008)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.040923-0
2013-01-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/63/1/158.html?itemId=/content/journal/ijsem/10.1099/ijs.0.040923-0&mimeType=html&fmt=ahah

References

  1. Albuquerque L., Rainey F. A., Chung A. P., Sunna A., Nobre M. F., Grote R., Antranikian G., da Costa M. S. ( 2000 ). Alicyclobacillus hesperidum sp. nov. and a related genomic species from solfataric soils of São Miguel in the Azores. . Int J Syst Evol Microbiol 50, 451457. [View Article] [PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [View Article] [PubMed]
    [Google Scholar]
  3. da Costa M. S., Albuquerque L., Nobre M. F., Wait R. ( 2011a ). The identification of polar lipids in prokaryotes. . In Methods in Microbiology (Taxonomy of Prokaryotes), vol. 38, pp. 165181. Edited by Rainey F. A., Oren A. . London:: Academic Press;. [View Article]
    [Google Scholar]
  4. da Costa M. S., Albuquerque L., Nobre M. F., Wait R. ( 2011b ). The identification of fatty acids in Bacteria. . In Methods in Microbiology (Taxonomy of Prokaryotes), vol. 38, pp. 183196. Edited by Rainey F. A., Oren A. . London:: Academic Press;. [View Article]
    [Google Scholar]
  5. da Costa M. S., Albuquerque L., Nobre M. F., Wait R. ( 2011c ). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. . In Methods in Microbiology (Taxonomy of Prokaryotes), vol. 38, pp. 197206. Edited by Rainey F. A., Oren A. . London:: Academic Press;. [View Article]
    [Google Scholar]
  6. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [View Article] [PubMed]
    [Google Scholar]
  7. Doetsch R. N. ( 1981 ). Determinative methods of light microscopy. . In Manual of Methods for General Microbiology, pp. 2133. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. H. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Felsenstein J. ( 1983 ). Parsimony in systematics: biological and statistical issues. . Annu Rev Ecol Syst 14, 313333. [View Article]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  10. Hatayama K., Shoun H., Ueda Y., Nakamura A. ( 2006 ). Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov.. Int J Syst Evol Microbiol 56, 25452551. [View Article] [PubMed]
    [Google Scholar]
  11. Huß V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [View Article]
    [Google Scholar]
  12. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;.
    [Google Scholar]
  13. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. & other authors ( 2004 ). ARB: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [View Article] [PubMed]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  15. Morgan F. J., Adams K. R., Priest F. G. ( 1979 ). A cultural method for the detection of pullulan–degrading enzymes in bacteria and its application to the genus Bacillus . . J Appl Bacteriol 46, 291294. [View Article] [PubMed]
    [Google Scholar]
  16. Pereira A. J. S. C., Neves L. J. P. F., Dias J. M. M., Campos A. B. A., Barbosa S. V. T. ( 2004 ). Evaluation of the radiological hazards from uranium mining and milling wastes (Urgeiriça – Central Portugal). . In Proceedings of the XI International Congress of the International Radiation Protection Association, pp. 10. Madrid:: Spanish Radiation Protection Society;.
    [Google Scholar]
  17. Rainey F. A., Ward-Rainey N., Kroppenstedt R. M., Stackebrandt E. ( 1996 ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov.. Int J Syst Bacteriol 46, 10881092. [View Article] [PubMed]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Schleifer K. H. ( 1985 ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18, 123156. [View Article]
    [Google Scholar]
  20. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  21. Smibert R. M., Krieg N. R. ( 1981 ). General characterization. . In Manual of Methods for General Bacteriology, pp. 411442. Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  22. Tomimura E., Zeman N. W., Frankiewicz J. R., Teague W. M. ( 1990 ). Description of Bacillus naganoensis sp. nov.. Int J Syst Bacteriol 40, 123125. [View Article] [PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.040923-0
Loading
/content/journal/ijsem/10.1099/ijs.0.040923-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error