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Volume 63, Issue Pt_2

sp. nov., a psychrophilic bacterium isolated from a marine sediment, and emended description of the genus Free

Abstract

A novel Gram-negative, rod-shaped, non-motile, psychrophilic bacterium, designated strain E4-9a, was isolated from a marine sediment sample collected at a depth of 276 m from Kongsfjorden, Svalbard, in the Arctic Ocean. The colony colour was golden yellow. Strain E4-9a was positive for amylase activity at 5 °C. The predominant fatty acids were iso-C G (21.8 %), anteiso-C (19.1 %), anteiso-C A (18.6 %), iso-C (13.8 %) and iso-C H (6.4 %). Strain E4-9a contained MK-6 as the major respiratory quinone. The polar lipids consisted of phosphatidylethanolamine, three unidentified aminolipids (AL1, AL4 and AL5), an unidentified phospholipid and four unidentified lipids (L1, L4 to L6). Based on 16S rRNA gene sequence similarity, it was ascertained that the closest related species to E4-9a were , and , with sequence similarity to the respective type strains of 98.5, 96.5 and 95.8 %. Phylogenetic analysis showed that strain E4-9a clustered with the type strain of and with those of and at distances of 1.5 and 4.8 % (98.5 and 95.2 % similarity), respectively. However, DNA–DNA hybridization with DJ3 showed 59 % relatedness with respect to strain E4-9a. The DNA G+C content of strain E4-9a was 29 mol%. Based on the results of DNA–DNA hybridization and phenotypic data, it appears that strain E4-9a represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E4-9a ( = CIP 110310  = KCTC 23612).

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Funding
This study was supported by the:
  • National Centre for Antarctic and Ocean Research, Goa
  • CSIR Network Project on Exploitation of India’s rich microbial diversity (Award NWP0006)
© 2013 IUMS
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2013-02-01
2024-04-25
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Lacinutrix himadriensis sp. nov., a psychrophilic bacterium isolated from a marine sediment, and emended description of the genus Lacinutrix
Int J Syst Evol Microbiol 63, 729 (2013); https://doi.org/10.1099/ijs.0.040907-0
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