1887

Abstract

A Gram-staining-negative, rod-shaped and non-motile bacterial strain, designated 12C25, was isolated from the crude-oil-degrading bacterial consortium enriched from mangrove sediment collected in Fujian Province, China. Optimal growth was observed at 25–28 °C, at pH 7.0 and in the presence of 2 % (w/v) NaCl + 2 % (w/v) KCl. Comparative 16S rRNA gene sequence analysis demonstrated that strain 12C25 shared the highest sequence similarity with members of the genus (97.7–93.9 %), exhibiting 97.7 % sequence similarity and 33.7±4 % DNA–DNA relatedness to SW-63. The DNA G+C content of strain 12C25 was 39.9 mol%. The dominant fatty acids were iso-C G, iso-C 3-OH, iso-C, C and iso-C 3-OH, and menaquinone with six isoprene units (MK-6) was the only respiratory quinone. On the basis of phenotypic data and phylogenetic inference, the novel strain belongs to the genus , but can readily be distinguished from known species of this genus and thus represents a novel species of the genus . The name sp. nov. is proposed and the type strain is 12C25 ( = CGMCC 1.11028 = MCCC 1F01096 = DSM 25030).

Funding
This study was supported by the:
  • , National Science Foundation of China , (Award 30930017, 41176093 and 40976069)
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2013-06-01
2020-12-02
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References

  1. Arun A. B., Chen W. M., Lai W. A., Chao J. H., Rekha P. D., Shen F. T., Singh S., Young C. C. ( 2009 ). Muricauda lutaonensis sp. nov., a moderate thermophile isolated from a coastal hot spring. . Int J Syst Evol Microbiol 59, 27382742. [CrossRef] [PubMed]
    [Google Scholar]
  2. Bruns A., Berthe-Corti L. ( 2011 ). Genus XXXV. Muricauda Bruns, Rohde and Berthe-Corti 2001, 2005VP emend. Yoon, Lee, Oh and Park 2005d, 1018. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 240245. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [CrossRef] [PubMed]
    [Google Scholar]
  4. Dong X.-Z., Cai M.-Y. (editors) ( 2001 ). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370398. Beijing:: Science Press; (in Chinese).
    [Google Scholar]
  5. Heatley N. G. ( 1944 ). A method for the assay of penicillin. . Biochem J 38, 6165.[PubMed]
    [Google Scholar]
  6. Hwang C. Y., Kim M. H., Bae G. D., Zhang G. I., Kim Y. H., Cho B. C. ( 2009 ). Muricauda olearia sp. nov., isolated from crude-oil-contaminated seawater, and emended description of the genus Muricauda. . Int J Syst Evol Microbiol 59, 18561861. [CrossRef] [PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  8. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  9. Lee S. Y., Park S., Oh T. K., Yoon J. H. ( 2012 ). Muricauda beolgyonensis sp. nov., isolated from a tidal flat. . Int J Syst Evol Microbiol 62, 11341139. [CrossRef] [PubMed]
    [Google Scholar]
  10. Liu C. L., Shao Z. Z. ( 2005 ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol 55, 11811186. [CrossRef] [PubMed]
    [Google Scholar]
  11. Liu H. J., Yang C. Y., Tian Y., Lin G. H., Zheng T. L. ( 2010 ). Screening of PAH-degrading bacteria in a mangrove swamp using PCR-RFLP. . Mar Poll Bull 60, 20562061. [CrossRef] [PubMed]
    [Google Scholar]
  12. Liu H. J., Yang C. Y., Tian Y., Lin G. H., Zheng T. L. ( 2011 ). Using population dynamics analysis by DGGE to design the bacterial consortium isolated from mangrove sediments for biodegradation of PAHs. . Int Biodeter Biodegr 65, 269275. [CrossRef]
    [Google Scholar]
  13. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297306. [CrossRef] [PubMed]
    [Google Scholar]
  14. Rzhetsky A., Nei M. ( 1992 ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9, 945967.
    [Google Scholar]
  15. Rzhetsky A., Nei M. ( 1993 ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. . Mol Biol Evol 10, 10731095.[PubMed]
    [Google Scholar]
  16. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  17. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  19. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  20. Yoon J. H., Lee M. H., Oh T. K., Park Y. H. ( 2005 ). Muricauda flavescens sp. nov. and Muricauda aquimarina sp. nov., isolated from a salt lake near Hwajinpo Beach of the East Sea in Korea, and emended description of the genus Muricauda . . Int J Syst Evol Microbiol 55, 10151019. [CrossRef] [PubMed]
    [Google Scholar]
  21. Yoon J. H., Kang S. J., Jung Y. T., Oh T. K. ( 2008 ). Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. . Int J Syst Evol Microbiol 58, 16031607. [CrossRef] [PubMed]
    [Google Scholar]
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